Viral pathogenesis in mice is similar for West Nile virus derived from mosquito and mammalian cells

Pei Yin Lim; Karen L. Louie; Linda M. Styer; Pei Yong Shi; Kristen A. Bernard

doi:10.1016/j.virol.2010.01.029

Viral pathogenesis in mice is similar for West Nile virus derived from mosquito and mammalian cells

Pei Yin Lim, Karen L. Louie, Linda M. Styer, Pei Yong Shi, Kristen A. Bernard

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

West Nile virus (WNV) is a mosquito-borne pathogen. During replication, WNV acquires different carbohydrates and lipid membranes, depending on its mosquito or vertebrate hosts. Consequently, WNV derived from mosquito and vertebrate cell lines differ in their infectivity for dendritic cells (DCs) and induction of type I interferon (IFN-α/β) in vitro. We evaluated the pathogenesis of WNV derived from mosquito (WNV^C6/36) and vertebrate (WNV^BHK) cell lines in mice. The tissue tropism, infectivity, clinical disease, and mortality did not differ for mice inoculated with WNV^C6/36 or WNV^BHK, and there were only minor differences in viral load and serum levels of IFN-α/β. The replication kinetics of WNV^C6/36 and WNV^BHK were equivalent in primary DCs and skin cells although primary DCs were more susceptible to WNV^C6/36 infection than to WNV^BHK infection, suggesting that less virus is produced per infected cell for WNV^C6/36. In conclusion, viral source has minimal effect on WNV pathogenesis in vivo.

Original language	English (US)
Pages (from-to)	93-103
Number of pages	11
Journal	Virology
Volume	400
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2010.01.029
State	Published - Apr 25 2010
Externally published	Yes

Keywords

Dendritic cells
Flavivirus
Mice
Mosquito
Pathogenesis
Skin
Type I interferon
Virus-host interaction
West Nile virus

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2010.01.029

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title = "Viral pathogenesis in mice is similar for West Nile virus derived from mosquito and mammalian cells",

abstract = "West Nile virus (WNV) is a mosquito-borne pathogen. During replication, WNV acquires different carbohydrates and lipid membranes, depending on its mosquito or vertebrate hosts. Consequently, WNV derived from mosquito and vertebrate cell lines differ in their infectivity for dendritic cells (DCs) and induction of type I interferon (IFN-α/β) in vitro. We evaluated the pathogenesis of WNV derived from mosquito (WNVC6/36) and vertebrate (WNVBHK) cell lines in mice. The tissue tropism, infectivity, clinical disease, and mortality did not differ for mice inoculated with WNVC6/36 or WNVBHK, and there were only minor differences in viral load and serum levels of IFN-α/β. The replication kinetics of WNVC6/36 and WNVBHK were equivalent in primary DCs and skin cells although primary DCs were more susceptible to WNVC6/36 infection than to WNVBHK infection, suggesting that less virus is produced per infected cell for WNVC6/36. In conclusion, viral source has minimal effect on WNV pathogenesis in vivo.",

keywords = "Dendritic cells, Flavivirus, Mice, Mosquito, Pathogenesis, Skin, Type I interferon, Virus-host interaction, West Nile virus",

author = "Lim, {Pei Yin} and Louie, {Karen L.} and Styer, {Linda M.} and Shi, {Pei Yong} and Bernard, {Kristen A.}",

note = "Funding Information: The work was supported in part by funds from the National Institute of Allergy and Infectious Disease , National Institutes of Health under contract N01-AI25490 and from the Centers for Disease Control and Prevention grant R01-CI000232-01 . The BSL-3 vivarium at the Wadsworth Center was used, which is funded in part as a core facility by NIH/NIAID U54-AI057158 (Northeast Biodefense Center).",

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doi = "10.1016/j.virol.2010.01.029",

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AU - Lim, Pei Yin

AU - Louie, Karen L.

AU - Styer, Linda M.

AU - Shi, Pei Yong

AU - Bernard, Kristen A.

N1 - Funding Information: The work was supported in part by funds from the National Institute of Allergy and Infectious Disease , National Institutes of Health under contract N01-AI25490 and from the Centers for Disease Control and Prevention grant R01-CI000232-01 . The BSL-3 vivarium at the Wadsworth Center was used, which is funded in part as a core facility by NIH/NIAID U54-AI057158 (Northeast Biodefense Center).

PY - 2010/4/25

Y1 - 2010/4/25

N2 - West Nile virus (WNV) is a mosquito-borne pathogen. During replication, WNV acquires different carbohydrates and lipid membranes, depending on its mosquito or vertebrate hosts. Consequently, WNV derived from mosquito and vertebrate cell lines differ in their infectivity for dendritic cells (DCs) and induction of type I interferon (IFN-α/β) in vitro. We evaluated the pathogenesis of WNV derived from mosquito (WNVC6/36) and vertebrate (WNVBHK) cell lines in mice. The tissue tropism, infectivity, clinical disease, and mortality did not differ for mice inoculated with WNVC6/36 or WNVBHK, and there were only minor differences in viral load and serum levels of IFN-α/β. The replication kinetics of WNVC6/36 and WNVBHK were equivalent in primary DCs and skin cells although primary DCs were more susceptible to WNVC6/36 infection than to WNVBHK infection, suggesting that less virus is produced per infected cell for WNVC6/36. In conclusion, viral source has minimal effect on WNV pathogenesis in vivo.

AB - West Nile virus (WNV) is a mosquito-borne pathogen. During replication, WNV acquires different carbohydrates and lipid membranes, depending on its mosquito or vertebrate hosts. Consequently, WNV derived from mosquito and vertebrate cell lines differ in their infectivity for dendritic cells (DCs) and induction of type I interferon (IFN-α/β) in vitro. We evaluated the pathogenesis of WNV derived from mosquito (WNVC6/36) and vertebrate (WNVBHK) cell lines in mice. The tissue tropism, infectivity, clinical disease, and mortality did not differ for mice inoculated with WNVC6/36 or WNVBHK, and there were only minor differences in viral load and serum levels of IFN-α/β. The replication kinetics of WNVC6/36 and WNVBHK were equivalent in primary DCs and skin cells although primary DCs were more susceptible to WNVC6/36 infection than to WNVBHK infection, suggesting that less virus is produced per infected cell for WNVC6/36. In conclusion, viral source has minimal effect on WNV pathogenesis in vivo.

KW - Dendritic cells

KW - Flavivirus

KW - Mice

KW - Mosquito

KW - Pathogenesis

KW - Skin

KW - Type I interferon

KW - Virus-host interaction

KW - West Nile virus

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Viral pathogenesis in mice is similar for West Nile virus derived from mosquito and mammalian cells

Abstract

Keywords

ASJC Scopus subject areas

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