TY - JOUR
T1 - Vector competence of aedes aegypti and aedes vittatus (Diptera: Culicidae) from Senegal and Cape Verde archipelago for West African lineages of chikungunya virus
AU - Diagne, Cheikh T.
AU - Faye, Oumar
AU - Guerbois, Mathilde
AU - Knight, Rachel
AU - Diallo, Diawo
AU - Faye, Ousmane
AU - Ba, Yamar
AU - Dia, Ibrahima
AU - Weaver, Scott C.
AU - Sall, Amadou A.
AU - Diallo, Mawlouth
N1 - Publisher Copyright:
© 2014 by The American Society of Tropical Medicine and Hygiene.
PY - 2014/9/1
Y1 - 2014/9/1
N2 - To assess the risk of emergence of chikungunya virus (CHIKV) in West Africa, vector competence of wildtype, urban, and non-urban Aedes aegypti and Ae. vittatus from Senegal and Cape Verde for CHIKV was investigated. Mosquitoes were fed orally with CHIKV isolates from mosquitoes (ArD30237), bats (CS13-288), and humans (HD180738). After 5, 10, and 15 days of incubation following an infectious blood meal, presence of CHIKV RNA was determined in bodies, legs/wings, and saliva using real-time reverse transcription-polymerase chain reaction. Aedes vittatus showed high susceptibility (50-100%) and early dissemination and transmission of all CHIKV strains tested. Aedes aegypti exhibited infection rates ranging from 0% to 50%. Aedes aegypti from Cape Verde and Kedougou, but not those from Dakar, showed the potential to transmit CHIKV in saliva. Analysis of biology and competence showed relatively high infective survival rates for Ae. vittatus and Ae. aegypti from Cape Verde, suggesting their efficient vector capacity in West Africa.
AB - To assess the risk of emergence of chikungunya virus (CHIKV) in West Africa, vector competence of wildtype, urban, and non-urban Aedes aegypti and Ae. vittatus from Senegal and Cape Verde for CHIKV was investigated. Mosquitoes were fed orally with CHIKV isolates from mosquitoes (ArD30237), bats (CS13-288), and humans (HD180738). After 5, 10, and 15 days of incubation following an infectious blood meal, presence of CHIKV RNA was determined in bodies, legs/wings, and saliva using real-time reverse transcription-polymerase chain reaction. Aedes vittatus showed high susceptibility (50-100%) and early dissemination and transmission of all CHIKV strains tested. Aedes aegypti exhibited infection rates ranging from 0% to 50%. Aedes aegypti from Cape Verde and Kedougou, but not those from Dakar, showed the potential to transmit CHIKV in saliva. Analysis of biology and competence showed relatively high infective survival rates for Ae. vittatus and Ae. aegypti from Cape Verde, suggesting their efficient vector capacity in West Africa.
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U2 - 10.4269/ajtmh.13-0627
DO - 10.4269/ajtmh.13-0627
M3 - Article
C2 - 25002293
AN - SCOPUS:84907017715
SN - 0002-9637
VL - 91
SP - 635
EP - 641
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 3
ER -