Vasoactive intestinal peptide enhances wound healing and proliferation of human bronchial epithelial cells

Cha Xiang Guan, Min Zhang, Xiao Qun Qin, Yan Ru Cui, Zi Qiang Luo, Hong Bo Bai, Xiang Fang

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


In the present study, we investigated the effects of vasoactive intestinal peptide (VIP) on wound healing of bronchial epithelium. Wound healing of the mechanical damaged human bronchial epithelial cells (HBEC) was observed in the absence or presence of VIP. Effects of VIP on chemotactic migration, cell proliferation of HBEC were also tested. HBEC chemotaxis was assessed by the blind well chamber technique, the cell cycle was determined by flow cytometry, and cell proliferation was determined by measuring the expression of proliferating cell nuclear antigen Ki67. Effects of VIP on epithelial E-cadherins protein and mRNA were also measured by immunohistochemistry and RT-PCR. The results showed that VIP accelerated the recovery of wound area of HBEC. VIP increased the migration and proliferation of HBEC, and these effects were blocked by a VPAC1 receptor antagonist. VIP also increased the expression of E-cadherin mRNA and protein in HBEC, suggesting that protective effects of VIP on wound healing may be related to its ability to increase the expression of E-cadherin. In conclusion, VIP has protective effects against human bronchial epithelial cell damage, and the beneficial effects of VIP might be mediated, at least in part, by VPAC1, and associated with increased expression of E-cadherin.

Original languageEnglish (US)
Pages (from-to)3107-3114
Number of pages8
Issue number12
StatePublished - Dec 2006
Externally publishedYes


  • Human bronchial epithelial cells
  • Proliferation
  • Vasoactive intestinal peptide
  • Wound healing

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience


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