TY - JOUR
T1 - Using MALDI-TOF MS to identify mosquitoes collected in Mali and their blood meals
AU - Tandina, Fatalmoudou
AU - Niaré, Sirama
AU - Laroche, Maureen
AU - Koné, Abdoulaye K.
AU - Diarra, Adama Z.
AU - Ongoiba, Abdoulaye
AU - Berenger, Jean Michel
AU - Doumbo, Ogobara K.
AU - Raoult, Didier
AU - Parola, Philippe
N1 - Publisher Copyright:
© 2018 Cambridge University Press.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently described as an innovative and effective tool for identifying arthropods and mosquito blood meal sources. To test this approach in the context of an entomological survey in the field, mosquitoes were collected from five ecologically distinct areas of Mali. We successfully analysed the blood meals from 651 mosquito abdomens crushed on Whatman filter paper (WFPs) in the field using MALDI-TOF MS. The legs of 826 mosquitoes were then submitted for MALDI-TOF MS analysis in order to identify the different mosquito species. Eight mosquito species were identified, including Anopheles gambiae Giles, Anopheles coluzzii, Anopheles arabiensis, Culex quinquefasciatus, Culex neavei, Culex perexiguus, Aedes aegypti and Aedes fowleri in Mali. The field mosquitoes for which MALDI-TOF MS did not provide successful identification were not previously available in our database. These specimens were subsequently molecularly identified. The WFP blood meal sources found in this study were matched against human blood (n = 619), chicken blood (n = 9), cow blood (n = 9), donkey blood (n = 6), dog blood (n = 5) and sheep blood (n = 3). This study reinforces the fact that MALDI-TOF MS is a promising tool for entomological surveys.
AB - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently described as an innovative and effective tool for identifying arthropods and mosquito blood meal sources. To test this approach in the context of an entomological survey in the field, mosquitoes were collected from five ecologically distinct areas of Mali. We successfully analysed the blood meals from 651 mosquito abdomens crushed on Whatman filter paper (WFPs) in the field using MALDI-TOF MS. The legs of 826 mosquitoes were then submitted for MALDI-TOF MS analysis in order to identify the different mosquito species. Eight mosquito species were identified, including Anopheles gambiae Giles, Anopheles coluzzii, Anopheles arabiensis, Culex quinquefasciatus, Culex neavei, Culex perexiguus, Aedes aegypti and Aedes fowleri in Mali. The field mosquitoes for which MALDI-TOF MS did not provide successful identification were not previously available in our database. These specimens were subsequently molecularly identified. The WFP blood meal sources found in this study were matched against human blood (n = 619), chicken blood (n = 9), cow blood (n = 9), donkey blood (n = 6), dog blood (n = 5) and sheep blood (n = 3). This study reinforces the fact that MALDI-TOF MS is a promising tool for entomological surveys.
KW - MALDI-TOF MS
KW - Mali
KW - Whatman
KW - blood meals
KW - field
KW - mosquito
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U2 - 10.1017/S0031182018000070
DO - 10.1017/S0031182018000070
M3 - Article
C2 - 29409547
AN - SCOPUS:85061162371
SN - 1060-1503
VL - 145
SP - 1170
EP - 1182
JO - Victorian Literature and Culture
JF - Victorian Literature and Culture
IS - 9
ER -