Using DNA barcoding to identify species within euphorbiaceae

Xiaohui Pang; Jingyuan Song; Yingjie Zhu; Caixiang Xie; Shilin Chen

doi:10.1055/s-0030-1249806

Using DNA barcoding to identify species within euphorbiaceae

Xiaohui Pang, Jingyuan Song, Yingjie Zhu, Caixiang Xie, Shilin Chen

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

In this study, we tested the applicability of four DNA regions (rbcL, matK, ITS, and ITS2) as barcodes for identifying species within Euphorbiaceae. Based on assessments of the specific genetic divergence, the DNA barcoding gap, and the ability for species discrimination, the present results affirmed that ITS/ITS2 is a potential barcode for the Euphorbiaceae species. This study also provided a large-scale test to evaluate the effectiveness of ITS/ITS2 for differentiating species within Euphorbiaceae. Of the 1183 plant samples collected from 871 species in 66 diverse genera, ITS/ITS2 successfully identified >90% and 100% of them at the species and genus levels, respectively. Therefore, our research indicates that use of the ITS/ITS2 region is a powerful technique for Euphorbiaceae identification.

Original language	English (US)
Pages (from-to)	1784-1786
Number of pages	3
Journal	Planta Medica
Volume	76
Issue number	15
DOIs	https://doi.org/10.1055/s-0030-1249806
State	Published - 2010
Externally published	Yes

Keywords

DNA barcoding
Euphorbiaceae
intra/interspecific genetic divergence
species identification

ASJC Scopus subject areas

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

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Cite this

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abstract = "In this study, we tested the applicability of four DNA regions (rbcL, matK, ITS, and ITS2) as barcodes for identifying species within Euphorbiaceae. Based on assessments of the specific genetic divergence, the DNA barcoding gap, and the ability for species discrimination, the present results affirmed that ITS/ITS2 is a potential barcode for the Euphorbiaceae species. This study also provided a large-scale test to evaluate the effectiveness of ITS/ITS2 for differentiating species within Euphorbiaceae. Of the 1183 plant samples collected from 871 species in 66 diverse genera, ITS/ITS2 successfully identified >90% and 100% of them at the species and genus levels, respectively. Therefore, our research indicates that use of the ITS/ITS2 region is a powerful technique for Euphorbiaceae identification.",

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author = "Xiaohui Pang and Jingyuan Song and Yingjie Zhu and Caixiang Xie and Shilin Chen",

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AU - Pang, Xiaohui

AU - Song, Jingyuan

AU - Zhu, Yingjie

AU - Xie, Caixiang

AU - Chen, Shilin

PY - 2010

Y1 - 2010

N2 - In this study, we tested the applicability of four DNA regions (rbcL, matK, ITS, and ITS2) as barcodes for identifying species within Euphorbiaceae. Based on assessments of the specific genetic divergence, the DNA barcoding gap, and the ability for species discrimination, the present results affirmed that ITS/ITS2 is a potential barcode for the Euphorbiaceae species. This study also provided a large-scale test to evaluate the effectiveness of ITS/ITS2 for differentiating species within Euphorbiaceae. Of the 1183 plant samples collected from 871 species in 66 diverse genera, ITS/ITS2 successfully identified >90% and 100% of them at the species and genus levels, respectively. Therefore, our research indicates that use of the ITS/ITS2 region is a powerful technique for Euphorbiaceae identification.

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KW - intra/interspecific genetic divergence

KW - species identification

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Using DNA barcoding to identify species within euphorbiaceae

Abstract

Keywords

ASJC Scopus subject areas

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