TY - JOUR
T1 - Uptake of 131I-metaiodobenzylguanidine by 6-23 rat medullary thyroid carcinoma
AU - Poston, Graeme J.
AU - Daniel Fawcett, H.
AU - Townsend, Courtney M.
AU - James, Elena
AU - Lieg, James L.
AU - Nusynowitz, Martin L.
AU - Thompson, James C.
N1 - Funding Information:
Acknowledgements Dr. Poston is in receipt of the Hurst Traveling Fellowship of the British Digestive Foundation, Ethicon Foundation travel grant and Wellcome Foundation travel grant. The authors would like to thank Cary W. Cooper, Ph.D., Department of Pharmacology and Toxicology, The University of Texas Medical Branch, for performing the CGRP radioimmunoassays and Mrs. Kelly Lee for the preparation of the manuscript.
PY - 1989
Y1 - 1989
N2 - Uptake of 131iodine-metaiodobenzylguanidine (131I-MIBG) by 6-23 rat medullary thyroid carcinoma (MTC), was studied in vitro and in vivo. In vitro, there was an 8-fold increase in 131I uptake by 6-23 cells when labeled with 131I-MIBG (131I 24±15 cpm/106 cells, 131I-MIBG 196±9 cpm/106 cells). MIBG uptake in vitro was the same at 4°C and 37°C. In contrast, (131I-MIBG uptake by PC-12 rat pheochromocytoma cells were 200 times greater (131I-MIBG 42,412±6,755 cpm/106 cells). 131I-MIBG uptake by rat MTC cells in vitro were of a comparable magnitude to the uptake of 131I-MIBG by rat ileal enterochromaffin cells (RIE-1) and mouse colon cancer cells (MC-26). In vivo, uptake of 131I-MIBG by 6-23 MTC tumor was considerably less than in the normal tissues (muscle, liver, spleen, kidney, adrenal and thyroid). Gamma camera studies of 131I-MIBG uptake by 6-23 MTC tumors growing in Wag-Rij rats were only transiently positive in 1 out of 4 rats studied. We conclude that 131I-MIBG is poorly taken up by rat medullary thyroid carcinoma and is an unpredictable marker for localization of rat MTC.
AB - Uptake of 131iodine-metaiodobenzylguanidine (131I-MIBG) by 6-23 rat medullary thyroid carcinoma (MTC), was studied in vitro and in vivo. In vitro, there was an 8-fold increase in 131I uptake by 6-23 cells when labeled with 131I-MIBG (131I 24±15 cpm/106 cells, 131I-MIBG 196±9 cpm/106 cells). MIBG uptake in vitro was the same at 4°C and 37°C. In contrast, (131I-MIBG uptake by PC-12 rat pheochromocytoma cells were 200 times greater (131I-MIBG 42,412±6,755 cpm/106 cells). 131I-MIBG uptake by rat MTC cells in vitro were of a comparable magnitude to the uptake of 131I-MIBG by rat ileal enterochromaffin cells (RIE-1) and mouse colon cancer cells (MC-26). In vivo, uptake of 131I-MIBG by 6-23 MTC tumor was considerably less than in the normal tissues (muscle, liver, spleen, kidney, adrenal and thyroid). Gamma camera studies of 131I-MIBG uptake by 6-23 MTC tumors growing in Wag-Rij rats were only transiently positive in 1 out of 4 rats studied. We conclude that 131I-MIBG is poorly taken up by rat medullary thyroid carcinoma and is an unpredictable marker for localization of rat MTC.
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U2 - 10.1016/0024-3205(89)90456-6
DO - 10.1016/0024-3205(89)90456-6
M3 - Article
C2 - 2786600
AN - SCOPUS:0024474402
SN - 0024-3205
VL - 44
SP - 1611
EP - 1616
JO - Life Sciences
JF - Life Sciences
IS - 21
ER -