Two-dimensional peptide mapping of fibronectins from bovine aortic endothelial cells and bovine plasma

Charles R. Birdwell; Allan R. Brasier; Lorna A. Taylor

doi:10.1016/0006-291X(80)90302-2

Two-dimensional peptide mapping of fibronectins from bovine aortic endothelial cells and bovine plasma

Charles R. Birdwell, Allan R. Brasier, Lorna A. Taylor

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We have made a comparison between plasma and endothelial cell fibronectin, since these cells are in intimate contact with plasma in vivo. Cellular and secreted fibronectins were purified from cloned lines of adult bovine aortic endothelial cells, and compared to purified bovine plasma fibronectin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional peptide mapping. When unreduced, all three fibronectins migrated on gels as single bands with M_r 440,000. After reduction, cellular and secreted fibronectins migrated on gels as single bands with M_r 220,000, but plasma fibronectin migrated as two bands with M_r 220,000 and 210,000. All three fibronectins, including the two subunits of plasma fibronectin, had identical structures by peptide mapping analysis.

Original language	English (US)
Pages (from-to)	574-581
Number of pages	8
Journal	Topics in Catalysis
Volume	97
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(80)90302-2
State	Published - 1980
Externally published	Yes

Keywords

ABAE
SDS
adult bovine aortic endothelial
sodium dodecyl sulfate

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Biophysics
Molecular Biology

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10.1016/0006-291X(80)90302-2

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title = "Two-dimensional peptide mapping of fibronectins from bovine aortic endothelial cells and bovine plasma",

abstract = "We have made a comparison between plasma and endothelial cell fibronectin, since these cells are in intimate contact with plasma in vivo. Cellular and secreted fibronectins were purified from cloned lines of adult bovine aortic endothelial cells, and compared to purified bovine plasma fibronectin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional peptide mapping. When unreduced, all three fibronectins migrated on gels as single bands with Mr 440,000. After reduction, cellular and secreted fibronectins migrated on gels as single bands with Mr 220,000, but plasma fibronectin migrated as two bands with Mr 220,000 and 210,000. All three fibronectins, including the two subunits of plasma fibronectin, had identical structures by peptide mapping analysis.",

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year = "1980",

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T1 - Two-dimensional peptide mapping of fibronectins from bovine aortic endothelial cells and bovine plasma

AU - Birdwell, Charles R.

AU - Brasier, Allan R.

AU - Taylor, Lorna A.

PY - 1980

Y1 - 1980

N2 - We have made a comparison between plasma and endothelial cell fibronectin, since these cells are in intimate contact with plasma in vivo. Cellular and secreted fibronectins were purified from cloned lines of adult bovine aortic endothelial cells, and compared to purified bovine plasma fibronectin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional peptide mapping. When unreduced, all three fibronectins migrated on gels as single bands with Mr 440,000. After reduction, cellular and secreted fibronectins migrated on gels as single bands with Mr 220,000, but plasma fibronectin migrated as two bands with Mr 220,000 and 210,000. All three fibronectins, including the two subunits of plasma fibronectin, had identical structures by peptide mapping analysis.

AB - We have made a comparison between plasma and endothelial cell fibronectin, since these cells are in intimate contact with plasma in vivo. Cellular and secreted fibronectins were purified from cloned lines of adult bovine aortic endothelial cells, and compared to purified bovine plasma fibronectin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional peptide mapping. When unreduced, all three fibronectins migrated on gels as single bands with Mr 440,000. After reduction, cellular and secreted fibronectins migrated on gels as single bands with Mr 220,000, but plasma fibronectin migrated as two bands with Mr 220,000 and 210,000. All three fibronectins, including the two subunits of plasma fibronectin, had identical structures by peptide mapping analysis.

KW - ABAE

KW - SDS

KW - adult bovine aortic endothelial

KW - sodium dodecyl sulfate

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Two-dimensional peptide mapping of fibronectins from bovine aortic endothelial cells and bovine plasma

Abstract

Keywords

ASJC Scopus subject areas

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