Abstract
The incidence of positive autocontrol test results with column agglutination technology is a concern. This study investigates the incidence and significance of positive autocontrols in the ID Micro Typing System™ (gel) and the Gamma ReACT™ (REACT). The study encompassed a total of 1021 randomly selected samples from patients and 95 samples from donors collected during 1 month. The autocontrol testing was carried out according to the manufacturer's instructions for the column agglutination tests. The tube method was carried out using low-ionic-strength solution (LISS). The direct antiglobulin test (DAT) was performed using the tube method, and further investigated with elution studies if warranted. Seventy-nine patient's samples (7.74%) had a positive autocontrol: the gel test, 72 (91.13%); ReACT, 21 (26.58%) and the tube method, 27 (34.18%). Of the 79 positive autocontrols, 44 samples had a negative DAT. Of the samples with positive DAT results, only one possessed a clinically significant antibody, anti-D. Moreover, the same sample also tested positive in all three methods. Column agglutination techniques have increased sensitivity for a positive autocontrol beyond the conventional tube method. However, ReACT and gel tests differ significantly in their frequency of positives. Investigation of the significance of a positive autocontrol in column agglutination technology when the conventional tube method is also positive is suggested.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 50-52 |
| Number of pages | 3 |
| Journal | Immunohematology |
| Volume | 17 |
| Issue number | 2 |
| State | Published - 2001 |
| Externally published | Yes |
Keywords
- Autocontrol testing
- ID Micro Typing System™
- ReACT™
ASJC Scopus subject areas
- Hematology
- Immunology and Allergy