TY - JOUR
T1 - Transforming growth factor-β inhibits rat intestinal cell growth by regulating cell cycle specific gene expression
AU - Ko, Tien C.
AU - Beauchamp, R. Daniel
AU - Townsend, Courtney M.
AU - Thompson, E. Aubrey
AU - Thompson, James C.
N1 - Funding Information:
From the Departments of Surgery (TCK, RDB, CMT, JCT) and Human Biological Chemistry and Genetics (RDB, EAT), The University of Texas Medical Branch Galveston, Texas. Thii work is supported by grants from the National Institutes of Health (PO1 DK 35608 and 5137 DK 15241), the American Cancer Society (CR57-I), Shriners Burns Institute (# 15860), the Walls Medical Research Foundation, and the James E. Thompson Memorial Fund. *Tien C. Ko, MD, is the recipient of The Society for Surgery of the Alimentary Tract Career Development Award. Requests for reprints should be addressed to R. Daniel Beauchamp, MD, Department of Surgery, E-33, The University of Texas Medical Branch, Galveston, Texas 77555-0533. Presented at the 34th Annual Meeting of The society for Surgery of the Alimentary Tract, Boston, Massachusetts, May 17-19,1993.
PY - 1994/1
Y1 - 1994/1
N2 - Transforming growth factor-β1 (TGF-β1) inhibits the growth of intestinal cells, but the mechanisms involved are unknown. Using a rat intestinal crypt cell line (IEC-6), we determined the site of action in the cell cycle that TGF-β1 acts to suppress proliferation. We also examined the effect of TGF-β1 on the expression of proliferation-associated "immediate early" genes (zif268, jun-B, c-myc) during the early g1 phase and the cdc2 gene during the transition from the G1 phase to the S phase of the cell cycle. Cell cycle progression was determined by incorporation of 3H-thymidine, and gene expression was analyzed by Northern blot analysis. We found that TGF-β1 acts to inhibit proliferation of rat intestinal crypt cells by blocking cell cycle progression at the middle g1 phase. The genes activated during g1 can be divided into TGF-β1 insensitive (zif268, jun-B, and c-myc) and TGF-β1 sensitive (the cdc2 gene). TGF-β1 suppresses the induction of the cdc2 gene during the G1 S transition without inhibiting the activation of immediate early genes during the early g1 phase.
AB - Transforming growth factor-β1 (TGF-β1) inhibits the growth of intestinal cells, but the mechanisms involved are unknown. Using a rat intestinal crypt cell line (IEC-6), we determined the site of action in the cell cycle that TGF-β1 acts to suppress proliferation. We also examined the effect of TGF-β1 on the expression of proliferation-associated "immediate early" genes (zif268, jun-B, c-myc) during the early g1 phase and the cdc2 gene during the transition from the G1 phase to the S phase of the cell cycle. Cell cycle progression was determined by incorporation of 3H-thymidine, and gene expression was analyzed by Northern blot analysis. We found that TGF-β1 acts to inhibit proliferation of rat intestinal crypt cells by blocking cell cycle progression at the middle g1 phase. The genes activated during g1 can be divided into TGF-β1 insensitive (zif268, jun-B, and c-myc) and TGF-β1 sensitive (the cdc2 gene). TGF-β1 suppresses the induction of the cdc2 gene during the G1 S transition without inhibiting the activation of immediate early genes during the early g1 phase.
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U2 - 10.1016/0002-9610(94)90048-5
DO - 10.1016/0002-9610(94)90048-5
M3 - Article
C2 - 8311125
AN - SCOPUS:0028168703
SN - 0002-9610
VL - 167
SP - 14
EP - 20
JO - The American Journal of Surgery
JF - The American Journal of Surgery
IS - 1
ER -