Transfer of platelet-derived growth factor-BB gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin

H. Kuwahara; A. T. Mitchell; M. D. Macklin; J. Zhao; D. Listengarten; L. G. Phillips

doi:10.1080/028443100750059066

Transfer of platelet-derived growth factor-BB gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin

H. Kuwahara, A. T. Mitchell, M. D. Macklin, J. Zhao, D. Listengarten, L. G. Phillips

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We have used an in vitro model of wound contraction, the fibroblast-populated collagen lattice, to examine the effect of platelet-derived growth factor BB (PDGF-BB) and PDGF-BB gene transfer by gene gun on the contraction of lattices composed of either diabetic or non-diabetic human fibroblasts. The area of collagen lattice and DNA synthesis were measured in 12 specimens. There were significant increases in lattice contraction with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB gene compared with control (p < 0.01). DNA synthesis of the non-diabetic and diabetic fibroblast lattices showed significantly increased incorporation of tritiated thymidine with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB compared with controls (p < 0.05). The effect of PDGF-BB gene transfer on diabetic and non-diabetic fibroblasts was similar to that of 20 ng/ml or less of PDGF-BB.

Original language	English (US)
Pages (from-to)	301-307
Number of pages	7
Journal	Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
Volume	34
Issue number	4
DOIs	https://doi.org/10.1080/028443100750059066
State	Published - 2000
Externally published	Yes

Keywords

Diabetes
Fibroblast
Gene gun
Gene transfer
PDGF

ASJC Scopus subject areas

General Medicine

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10.1080/028443100750059066

Cite this

Kuwahara, H., Mitchell, A. T., Macklin, M. D., Zhao, J., Listengarten, D., & Phillips, L. G. (2000). Transfer of platelet-derived growth factor-BB gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin. Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery, 34(4), 301-307. https://doi.org/10.1080/028443100750059066

Transfer of platelet-derived growth factor-BB gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin. / Kuwahara, H.; Mitchell, A. T.; Macklin, M. D. et al.
In: Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery, Vol. 34, No. 4, 2000, p. 301-307.

Research output: Contribution to journal › Article › peer-review

Kuwahara, H, Mitchell, AT, Macklin, MD, Zhao, J, Listengarten, D & Phillips, LG 2000, 'Transfer of platelet-derived growth factor-BB gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin', Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery, vol. 34, no. 4, pp. 301-307. https://doi.org/10.1080/028443100750059066

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abstract = "We have used an in vitro model of wound contraction, the fibroblast-populated collagen lattice, to examine the effect of platelet-derived growth factor BB (PDGF-BB) and PDGF-BB gene transfer by gene gun on the contraction of lattices composed of either diabetic or non-diabetic human fibroblasts. The area of collagen lattice and DNA synthesis were measured in 12 specimens. There were significant increases in lattice contraction with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB gene compared with control (p < 0.01). DNA synthesis of the non-diabetic and diabetic fibroblast lattices showed significantly increased incorporation of tritiated thymidine with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB compared with controls (p < 0.05). The effect of PDGF-BB gene transfer on diabetic and non-diabetic fibroblasts was similar to that of 20 ng/ml or less of PDGF-BB.",

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AU - Zhao, J.

AU - Listengarten, D.

AU - Phillips, L. G.

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Transfer of platelet-derived growth factor-BB gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin

Abstract

Keywords

ASJC Scopus subject areas

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