TY - JOUR
T1 - Transcriptome analysis of the Ophiocordyceps sinensis fruiting body reveals putative genes involved in fruiting body development and cordycepin biosynthesis
AU - Xiang, Li
AU - Li, Ying
AU - Zhu, Yingjie
AU - Luo, Hongmei
AU - Li, Chunfang
AU - Xu, Xiaolan
AU - Sun, Chao
AU - Song, Jingyuan
AU - Shi, Linchun
AU - He, Liu
AU - Sun, Wei
AU - Chen, Shilin
PY - 2014/1
Y1 - 2014/1
N2 - Ophiocordyceps sinensis is a highly valuable and popular medicinal fungus used as a tonic and roborant for thousands of years in traditional Asian medicine. However, unsustainable harvesting practices have endangered this species and very little is known about its developmental programming, its biochemistry and genetics. To begin to address this, the transcriptome of the medicinal O. sinensis fruiting body was analyzed by high-throughput. In this O. sinensis 454-EST dataset, four mating type genes and 121 genes that may be involved in fruiting body development, especially in signal transduction and transcription regulation, were discovered. Moreover, a model was developed for the synthesis of the primary medicinal compound, cordycepin, and the putative biosynthetic enzymes identified. This transcriptome dataset provides a significant new resource for gene discovery in O. sinensis and dissection of its valuable biosynthetic and developmental pathways.
AB - Ophiocordyceps sinensis is a highly valuable and popular medicinal fungus used as a tonic and roborant for thousands of years in traditional Asian medicine. However, unsustainable harvesting practices have endangered this species and very little is known about its developmental programming, its biochemistry and genetics. To begin to address this, the transcriptome of the medicinal O. sinensis fruiting body was analyzed by high-throughput. In this O. sinensis 454-EST dataset, four mating type genes and 121 genes that may be involved in fruiting body development, especially in signal transduction and transcription regulation, were discovered. Moreover, a model was developed for the synthesis of the primary medicinal compound, cordycepin, and the putative biosynthetic enzymes identified. This transcriptome dataset provides a significant new resource for gene discovery in O. sinensis and dissection of its valuable biosynthetic and developmental pathways.
KW - Cordycepin
KW - Expressed sequence tag
KW - Ophiocordyceps sinensis
KW - Transcriptome
UR - http://www.scopus.com/inward/record.url?scp=84895865945&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84895865945&partnerID=8YFLogxK
U2 - 10.1016/j.ygeno.2014.01.002
DO - 10.1016/j.ygeno.2014.01.002
M3 - Article
C2 - 24440419
AN - SCOPUS:84895865945
SN - 0888-7543
VL - 103
SP - 154
EP - 159
JO - Genomics
JF - Genomics
IS - 1
ER -