Toxoplasma gondii: Protective immunity against toxoplasmosis with recombinant actin depolymerizing factor protein in BALB/c mice

Xiangsheng Huang; Jianhua Li; Guocai Zhang; Pengtao Gong; Ju Yang; Xichen Zhang

doi:10.1016/j.exppara.2012.01.001

Toxoplasma gondii: Protective immunity against toxoplasmosis with recombinant actin depolymerizing factor protein in BALB/c mice

Xiangsheng Huang, Jianhua Li, Guocai Zhang, Pengtao Gong, Ju Yang, Xichen Zhang

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Toxoplasmosis is one of the most world-wide spread zoonosis representing a very serious clinical and veterinary problem. There is still need for vaccines for toxoplasmosis. In the present study, we evaluated the protective efficacy of a recombinant actin depolymerizing factor (ADF) subunit vaccine against Toxoplasma gondii infection in BALB/c mice. The recombinant T. gondii ADF protein (rADF) was expressed in Escherichia coli and used as antigens for BALB/c mice immunization. The results indicated that specific antibody and the increased percentage of CD4 ⁺ T lymphocyte were found in vaccinated BALB/c mice with rADF, when compared with adjuvant or PBS groups. After challenged with T. gondii (RH strain) tachyzoites, the survival time of the mice in rADF group was longer than those in the control group. The numbers of brain cysts of the mice in rADF group reduced significantly when compared with those in control groups (P<0.05), and the rate of reduction could reach to around 30%. These results suggest that rADF can generate protective immunity against T. gondii infection in BALB/c mice.

Original language	English (US)
Pages (from-to)	218-222
Number of pages	5
Journal	Experimental Parasitology
Volume	130
Issue number	3
DOIs	https://doi.org/10.1016/j.exppara.2012.01.001
State	Published - Mar 2012
Externally published	Yes

Keywords

Actin depolymerizing factor
BALB/c mice
Recombinant protein
Toxoplasma gondii

ASJC Scopus subject areas

Parasitology
Immunology
Infectious Diseases

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10.1016/j.exppara.2012.01.001

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title = "Toxoplasma gondii: Protective immunity against toxoplasmosis with recombinant actin depolymerizing factor protein in BALB/c mice",

abstract = "Toxoplasmosis is one of the most world-wide spread zoonosis representing a very serious clinical and veterinary problem. There is still need for vaccines for toxoplasmosis. In the present study, we evaluated the protective efficacy of a recombinant actin depolymerizing factor (ADF) subunit vaccine against Toxoplasma gondii infection in BALB/c mice. The recombinant T. gondii ADF protein (rADF) was expressed in Escherichia coli and used as antigens for BALB/c mice immunization. The results indicated that specific antibody and the increased percentage of CD4 + T lymphocyte were found in vaccinated BALB/c mice with rADF, when compared with adjuvant or PBS groups. After challenged with T. gondii (RH strain) tachyzoites, the survival time of the mice in rADF group was longer than those in the control group. The numbers of brain cysts of the mice in rADF group reduced significantly when compared with those in control groups (P<0.05), and the rate of reduction could reach to around 30%. These results suggest that rADF can generate protective immunity against T. gondii infection in BALB/c mice.",

keywords = "Actin depolymerizing factor, BALB/c mice, Recombinant protein, Toxoplasma gondii",

author = "Xiangsheng Huang and Jianhua Li and Guocai Zhang and Pengtao Gong and Ju Yang and Xichen Zhang",

note = "Funding Information: This work was supported by High Technology Research and Development Program (863) of China (No. 2006AA10A207 ), National Key Technology R&D Program of China (No. 2007BAD40B05 ). ",

year = "2012",

month = mar,

doi = "10.1016/j.exppara.2012.01.001",

language = "English (US)",

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T2 - Protective immunity against toxoplasmosis with recombinant actin depolymerizing factor protein in BALB/c mice

AU - Huang, Xiangsheng

AU - Li, Jianhua

AU - Zhang, Guocai

AU - Gong, Pengtao

AU - Yang, Ju

AU - Zhang, Xichen

N1 - Funding Information: This work was supported by High Technology Research and Development Program (863) of China (No. 2006AA10A207 ), National Key Technology R&D Program of China (No. 2007BAD40B05 ).

PY - 2012/3

Y1 - 2012/3

N2 - Toxoplasmosis is one of the most world-wide spread zoonosis representing a very serious clinical and veterinary problem. There is still need for vaccines for toxoplasmosis. In the present study, we evaluated the protective efficacy of a recombinant actin depolymerizing factor (ADF) subunit vaccine against Toxoplasma gondii infection in BALB/c mice. The recombinant T. gondii ADF protein (rADF) was expressed in Escherichia coli and used as antigens for BALB/c mice immunization. The results indicated that specific antibody and the increased percentage of CD4 + T lymphocyte were found in vaccinated BALB/c mice with rADF, when compared with adjuvant or PBS groups. After challenged with T. gondii (RH strain) tachyzoites, the survival time of the mice in rADF group was longer than those in the control group. The numbers of brain cysts of the mice in rADF group reduced significantly when compared with those in control groups (P<0.05), and the rate of reduction could reach to around 30%. These results suggest that rADF can generate protective immunity against T. gondii infection in BALB/c mice.

AB - Toxoplasmosis is one of the most world-wide spread zoonosis representing a very serious clinical and veterinary problem. There is still need for vaccines for toxoplasmosis. In the present study, we evaluated the protective efficacy of a recombinant actin depolymerizing factor (ADF) subunit vaccine against Toxoplasma gondii infection in BALB/c mice. The recombinant T. gondii ADF protein (rADF) was expressed in Escherichia coli and used as antigens for BALB/c mice immunization. The results indicated that specific antibody and the increased percentage of CD4 + T lymphocyte were found in vaccinated BALB/c mice with rADF, when compared with adjuvant or PBS groups. After challenged with T. gondii (RH strain) tachyzoites, the survival time of the mice in rADF group was longer than those in the control group. The numbers of brain cysts of the mice in rADF group reduced significantly when compared with those in control groups (P<0.05), and the rate of reduction could reach to around 30%. These results suggest that rADF can generate protective immunity against T. gondii infection in BALB/c mice.

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KW - Toxoplasma gondii

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Toxoplasma gondii: Protective immunity against toxoplasmosis with recombinant actin depolymerizing factor protein in BALB/c mice

Abstract

Keywords

ASJC Scopus subject areas

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