Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses

K. Reddisiva Prasanth; Minato Hirano; W. Samuel Fagg; Eileen T. McAnarney; Chao Shan; Xuping Xie; Adam Hage; Colette A. Pietzsch; Alexander Bukreyev; Ricardo Rajsbaum; Pei Yong Shi; Mark T. Bedford; Shelton S. Bradrick; Vineet Menachery; Mariano A. Garcia-Blanco

doi:10.1016/j.antiviral.2020.104874

Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses

K. Reddisiva Prasanth, Minato Hirano, W. Samuel Fagg, Eileen T. McAnarney, Chao Shan, Xuping Xie, Adam Hage, Colette A. Pietzsch, Alexander Bukreyev, Ricardo Rajsbaum, Pei Yong Shi, Mark T. Bedford, Shelton S. Bradrick, Vineet Menachery, Mariano A. Garcia-Blanco

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.

Original language	English (US)
Article number	104874
Journal	Antiviral research
Volume	182
DOIs	https://doi.org/10.1016/j.antiviral.2020.104874
State	Published - Oct 2020

Keywords

Dengue
Host factor
Positive stranded RNA viruses
SARS-CoV-2
Topoisomerase III-ß (TOP3B)

ASJC Scopus subject areas

Pharmacology
Virology

Access to Document

10.1016/j.antiviral.2020.104874

Cite this

Prasanth, K. R., Hirano, M., Fagg, W. S., McAnarney, E. T., Shan, C., Xie, X., Hage, A., Pietzsch, C. A., Bukreyev, A., Rajsbaum, R., Shi, P. Y., Bedford, M. T., Bradrick, S. S., Menachery, V., & Garcia-Blanco, M. A. (2020). Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses. Antiviral research, 182, Article 104874. https://doi.org/10.1016/j.antiviral.2020.104874

@article{eb3c4b1dad534322a4a73c961eb22d2a,

title = "Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses",

abstract = "Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.",

keywords = "Dengue, Host factor, Positive stranded RNA viruses, SARS-CoV-2, Topoisomerase III-{\ss} (TOP3B)",

author = "Prasanth, {K. Reddisiva} and Minato Hirano and Fagg, {W. Samuel} and McAnarney, {Eileen T.} and Chao Shan and Xuping Xie and Adam Hage and Pietzsch, {Colette A.} and Alexander Bukreyev and Ricardo Rajsbaum and Shi, {Pei Yong} and Bedford, {Mark T.} and Bradrick, {Shelton S.} and Vineet Menachery and Garcia-Blanco, {Mariano A.}",

note = "Publisher Copyright: {\textcopyright} 2020 The Author(s)",

year = "2020",

month = oct,

doi = "10.1016/j.antiviral.2020.104874",

language = "English (US)",

volume = "182",

journal = "Antiviral research",

issn = "0166-3542",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses

AU - Prasanth, K. Reddisiva

AU - Hirano, Minato

AU - Fagg, W. Samuel

AU - McAnarney, Eileen T.

AU - Shan, Chao

AU - Xie, Xuping

AU - Hage, Adam

AU - Pietzsch, Colette A.

AU - Bukreyev, Alexander

AU - Rajsbaum, Ricardo

AU - Shi, Pei Yong

AU - Bedford, Mark T.

AU - Bradrick, Shelton S.

AU - Menachery, Vineet

AU - Garcia-Blanco, Mariano A.

PY - 2020/10

Y1 - 2020/10

N2 - Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.

AB - Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.

KW - Dengue

KW - Host factor

KW - Positive stranded RNA viruses

KW - SARS-CoV-2

KW - Topoisomerase III-ß (TOP3B)

UR - http://www.scopus.com/inward/record.url?scp=85089277326&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85089277326&partnerID=8YFLogxK

U2 - 10.1016/j.antiviral.2020.104874

DO - 10.1016/j.antiviral.2020.104874

M3 - Article

C2 - 32735900

AN - SCOPUS:85089277326

SN - 0166-3542

VL - 182

JO - Antiviral research

JF - Antiviral research

M1 - 104874

ER -

Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this