Three-Dimensional NOESY-NOESY Hybrid-Hybrid Matrix Refinement of a DNA Three-Way Junction

Varatharasa Thiviyanthan, Nishantha Illangasekare, Elliott Gozansky, Frank Zhu, Neocles B. Leontis, Bruce A. Luxon, David G. Gorenstein

Research output: Contribution to journalArticlepeer-review

Abstract

A computationally efficient hybrid-hybrid relaxation matrix refinement methodology using 3D NOESY-NOESY data is described. The methodology was tested on simulated data, derived from the Dickerson dodecamer, and used to refine the structure of a DNA three way junction from experimentally determined 3D NOESY-NOESY volume data. Our results indicate that the 3D hybrid-hybrid MORASS methodology, by combining the spectral dispersion of 3D NOESY-NOESY spectroscopy and the computational efficiency of 2D refinement programs, provides an accurate and robust means for structure determination of large biomolecules.

Original languageEnglish (US)
Pages (from-to)167-180
Number of pages14
JournalACS Symposium Series
Volume682
StatePublished - 1998
Externally publishedYes

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering

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