Abstract
A 2.15-kb PstI fragment of the 140-megadalton (MDa) plasmid of Shigella flexneri which encodes Congo red (CR) dye binding activity was isolated by recombinant techniques. The recombinant plasmid was shown to encode the structural gene for CR binding but failed to restore the ability of noninvasive strains to penetrate HeLa cell monolayers. A partial restriction map was constructed and polypeptides specified by the 140-MDa insert DNA identified in a minicell system. The putative polypeptide responsible for CR binding activity was identified by its thermoregulated expression in minicells. The Mr of the putative CR binding protein was 24000.
Original language | English (US) |
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Pages (from-to) | 281-286 |
Number of pages | 6 |
Journal | FEMS Microbiology Letters |
Volume | 28 |
Issue number | 3 |
DOIs | |
State | Published - Jul 1985 |
Externally published | Yes |
Keywords
- HeLa cell invasion
- minicells
- restriction maṕping
- virulence plasmid
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics