The role of oxidative stress in the developmental origin of adult hypertension

Labib M. Ghulmiyyah, Maged Costantine, Huaizhi Yin, Esther Tamayo, Shannon M. Clark, Gary Hankins, George Saade, Monica Longo

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Objective: To determine whether oxidative stress plays a role in the development of hypertension using a mouse model of fetal programming induced by endothelial nitric oxide synthase deficiency. Study Design: Homozygous nitric oxide synthase knockout and wild type mice were cross-bred producing maternal (endothelial nitric oxide synthase+pat/-mat) and paternal (endothelial nitric oxide synthase+mat/-pat) heterozygous offspring. RNA from liver and kidney tissues of female pups were obtained at 14 weeks of age. Relative expression of the heat shock protein-B6, peroxiredoxin-3, superoxide dismutase-1, peroxisome proliferator-activated receptor gamma, nitric oxide synthase-1 and -2 were determined. Results: In the kidneys, expression of nitric oxide synthase-2, peroxiredoxin-3, heat shock protein-B6, and superoxide dismutase-1 was up-regulated in endothelial nitric oxide synthase +pat/-mat but not in endothelial nitric oxide synthase +mat/-pat compared with wild type offspring. In the liver, there were no significant differences in the expression of nitric oxide synthase-1, nitric oxide synthase-2, peroxiredoxin, superoxide dismutase-1, or peroxisome proliferator-activated receptor gamma; however, heat shock protein-B6 was down-regulated in both heterozygotes offspring compared with wild type. Conclusion: The intrauterine environment alters oxidative pathways gene expression in the kidneys of offspring, which may be a mechanism in the development of adult hypertension.

Original languageEnglish (US)
Pages (from-to)155.e7-155.e11
JournalAmerican journal of obstetrics and gynecology
Issue number2
StatePublished - Aug 2011
Externally publishedYes


  • fetal programming
  • gene expression
  • oxidative stress

ASJC Scopus subject areas

  • Obstetrics and Gynecology


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