The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool

Xue Jie Yu; Patricia Crocquet-Valdes; Louis C. Cullman; David H. Walker

doi:10.1128/jcm.34.11.2853-2855.1996

The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool

Xue Jie Yu, Patricia Crocquet-Valdes, Louis C. Cullman, David H. Walker

Pathology

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Abstract

DNA encoding two repeat units of the 120-kDa protein of Ehrlichia chaffensis was cloned into the expression vector pGEX and expressed in Escherichia coli. The sensitivity and specificity of a dot blot assay for detection of human antibodies with the recombinant protein were 86 and 100%, respectively, compared with an indirect immunofluorescence assay.

Original language	English (US)
Pages (from-to)	2853-2855
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	34
Issue number	11
DOIs	https://doi.org/10.1128/jcm.34.11.2853-2855.1996
State	Published - Nov 1996

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/jcm.34.11.2853-2855.1996

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abstract = "DNA encoding two repeat units of the 120-kDa protein of Ehrlichia chaffensis was cloned into the expression vector pGEX and expressed in Escherichia coli. The sensitivity and specificity of a dot blot assay for detection of human antibodies with the recombinant protein were 86 and 100%, respectively, compared with an indirect immunofluorescence assay.",

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AB - DNA encoding two repeat units of the 120-kDa protein of Ehrlichia chaffensis was cloned into the expression vector pGEX and expressed in Escherichia coli. The sensitivity and specificity of a dot blot assay for detection of human antibodies with the recombinant protein were 86 and 100%, respectively, compared with an indirect immunofluorescence assay.

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The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool

Abstract

ASJC Scopus subject areas

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