TY - JOUR
T1 - The presence of Ia antigen on human peripheral blood T cells and T-cell subsets
T2 - Analysis with monoclonal antibodies and the fluorescence-activated cell sorter
AU - Ceuppens, Jan L.
AU - Goodwin, James S.
AU - Searles, Robert P.
N1 - Funding Information:
’ This work was supported in part by Grants (AG-01245) and (AM-00793) from the National Institutes of Health. 2 Supported by a grant from the Belgian National Fund of Scientific Research. Present address: Division of Clinical Immunology, Department of Medicine, St. Rafael Hospital, University of Louvain, Louvain, Belgium. 3 To whom requests for reprints should be addressed.
PY - 1981/11/1
Y1 - 1981/11/1
N2 - Peripheral blood T cells from normal donors, from newborns, and from patients with rheumatoid arthritis were analyzed for the presence of surface Ia antigens using monoclonal anti-Ia reagents and a fluorescence-activated cell sorter. Two out of four monoclonal anti-Ia antibodies detected Ia antigen on 2-22% (mean 7.4 ± 5.9%) of normal E rosette (+) cells. A subpopulation of E rosette (+) cells thus expresses an Ia molecule with antigenic determinants similar to the Ia on B cells. The staining is weak in comparison to the staining with anti-Ia of B cells, but could not be blocked by preincubation of the T cells with aggregated human IgG. Confirming a previous report by Yu et al. (D. T. Y. Yu, R. J. Winchester, S. M. Fu, A. Gibefsky, H. S. Ko, and H. G. Kunkel, J. Exp. Med., 151, 91, 1980) in rheumatoid arthritis more Ia-positive T cells were detected (mean 17.4 ± 12.5%) that also express a higher number of Ia molecules on their surface. On the contrary, the number of Ia(+) T cells was decreased in cord blood. Both helper T cells [OKT4(+)] and suppressor T cells [OKT8(+)] contain a subfraction that is Ia(+), as evidenced by additive staining and two-color immunofluorescence experiments. In RA, an increase in Ia(+) cytotoxic/suppressor cells is mainly responsible for the higher proportion of Ia(+) T cells. We have previously identified a subpopulation of T cells staining with both OKT8 and OKM1, a marker thought to be specific for cells of myelomonocytic lineage. We now report that this OKT8(+) OKM1(+) overlap population is almost entirely Ia(+) and therefore could represent an activated cell type. However, Ia antigen was also detected on OKT3(+) cells after removing OKM1(+) E rosette (+) cells.
AB - Peripheral blood T cells from normal donors, from newborns, and from patients with rheumatoid arthritis were analyzed for the presence of surface Ia antigens using monoclonal anti-Ia reagents and a fluorescence-activated cell sorter. Two out of four monoclonal anti-Ia antibodies detected Ia antigen on 2-22% (mean 7.4 ± 5.9%) of normal E rosette (+) cells. A subpopulation of E rosette (+) cells thus expresses an Ia molecule with antigenic determinants similar to the Ia on B cells. The staining is weak in comparison to the staining with anti-Ia of B cells, but could not be blocked by preincubation of the T cells with aggregated human IgG. Confirming a previous report by Yu et al. (D. T. Y. Yu, R. J. Winchester, S. M. Fu, A. Gibefsky, H. S. Ko, and H. G. Kunkel, J. Exp. Med., 151, 91, 1980) in rheumatoid arthritis more Ia-positive T cells were detected (mean 17.4 ± 12.5%) that also express a higher number of Ia molecules on their surface. On the contrary, the number of Ia(+) T cells was decreased in cord blood. Both helper T cells [OKT4(+)] and suppressor T cells [OKT8(+)] contain a subfraction that is Ia(+), as evidenced by additive staining and two-color immunofluorescence experiments. In RA, an increase in Ia(+) cytotoxic/suppressor cells is mainly responsible for the higher proportion of Ia(+) T cells. We have previously identified a subpopulation of T cells staining with both OKT8 and OKM1, a marker thought to be specific for cells of myelomonocytic lineage. We now report that this OKT8(+) OKM1(+) overlap population is almost entirely Ia(+) and therefore could represent an activated cell type. However, Ia antigen was also detected on OKT3(+) cells after removing OKM1(+) E rosette (+) cells.
UR - http://www.scopus.com/inward/record.url?scp=0019793480&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019793480&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(81)90480-9
DO - 10.1016/0008-8749(81)90480-9
M3 - Article
C2 - 6458364
AN - SCOPUS:0019793480
SN - 0008-8749
VL - 64
SP - 277
EP - 292
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -