TY - JOUR
T1 - The nucleotide sequence of the RAD3 gene of Saccharomyces cerevisiae
T2 - A potential adenine nucleotide binding amino acid sequence and a nonessential acidic carboxyl terminal region
AU - Reynolds, Paul
AU - Higgins, David R.
AU - Prakash, Louise
AU - Prakash, Satya
N1 - Funding Information:
ACKNOWLEDGEMENTS We thank Alan Morrison for helpful discussions and Eugene LeClerc, Nancy Istock, and David Barker for advice on DNA sequencing. This work was supported by grants CA35035 to S.P. and GM19261 to L.P. from NIH, and contract No. DE-AC02-76EV03490 with the U.S. Department of Energy at the University of Rochester Department of Radiation Biology and Biophysics and has been assigned number DOE/EV/03490-2472.
PY - 1985/4/11
Y1 - 1985/4/11
N2 - The RAD3 gene of Saccharomyces cerevisiae is required for excision of pyrimidine dimers and is essential for viability. We present the nucleotide sequence of the RAD3 protein coding region and its flanking regions, and the deduced primary structure of the RAD3 protein. In addition, we have mapped the 5′ end of RAD3 mRNA. The predicted RAD3 protein contains 778 amino acids with a calculated molecular weight of 89, 779. A segment of the RAD3 protein shares homology with several adenine nucleotide binding proteins, suggesting that RAD3 protein may react with ATP. The twenty carboxyl terminal amino acids of RAD3 protein are predominantly acidic; however, deletion of this acidic region has no obvious effect on viability or DNA repair
AB - The RAD3 gene of Saccharomyces cerevisiae is required for excision of pyrimidine dimers and is essential for viability. We present the nucleotide sequence of the RAD3 protein coding region and its flanking regions, and the deduced primary structure of the RAD3 protein. In addition, we have mapped the 5′ end of RAD3 mRNA. The predicted RAD3 protein contains 778 amino acids with a calculated molecular weight of 89, 779. A segment of the RAD3 protein shares homology with several adenine nucleotide binding proteins, suggesting that RAD3 protein may react with ATP. The twenty carboxyl terminal amino acids of RAD3 protein are predominantly acidic; however, deletion of this acidic region has no obvious effect on viability or DNA repair
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U2 - 10.1093/nar/13.7.2357
DO - 10.1093/nar/13.7.2357
M3 - Article
C2 - 2987851
AN - SCOPUS:0022423251
SN - 0305-1048
VL - 13
SP - 2357
EP - 2372
JO - Nucleic acids research
JF - Nucleic acids research
IS - 7
ER -