TY - JOUR
T1 - The human Werner syndrome protein stimulates repair of oxidative DNA base damage by the DNA glycosylase NEIL1
AU - Das, Aditi
AU - Boldogh, Istvan
AU - Jae, Wan Lee
AU - Harrigan, Jeanine A.
AU - Hegde, Muralidhar L.
AU - Piotrowski, Jason
AU - Pinto, Nadja De Souza
AU - Ramos, William
AU - Greenberg, Marc M.
AU - Hazra, Tapas K.
AU - Mitra, Sankar
AU - Bohr, Vilhelm A.
PY - 2007/9/7
Y1 - 2007/9/7
N2 - The mammalian DNA glycosylase, NEIL1, specific for repair of oxidatively damaged bases in the genome via the base excision repair pathway, is activated by reactive oxygen species and prevents toxicity due to radiation. We show here that the Werner syndrome protein (WRN), a member of the RecQ family of DNA helicases, associates with NEIL1 in the early damage-sensing step of base excision repair. WRN stimulates NEIL1 in excision of oxidative lesions from bubble DNA substrates. The binary interaction between NEIL1 and WRN (K D = 60 nM) involves C-terminal residues 288-349 of NEIL1 and the RecQ C-terminal (RQC) region of WRN, and is independent of the helicase activity WRN. Exposure to oxidative stress enhances the NEIL-WRN association concomitant with their strong nuclear co-localization. WRN-depleted cells accumulate some prototypical oxidized bases (e.g. 8-oxoguanine, FapyG, and FapyA) indicating a physiological function of WRN in oxidative damage repair in mammalian genomes. Interestingly, WRN deficiency does not have an additive effect on in vivo damage accumulation in NEIL1 knockdown cells suggesting that WRN participates in the same repair pathway as NEIL1.
AB - The mammalian DNA glycosylase, NEIL1, specific for repair of oxidatively damaged bases in the genome via the base excision repair pathway, is activated by reactive oxygen species and prevents toxicity due to radiation. We show here that the Werner syndrome protein (WRN), a member of the RecQ family of DNA helicases, associates with NEIL1 in the early damage-sensing step of base excision repair. WRN stimulates NEIL1 in excision of oxidative lesions from bubble DNA substrates. The binary interaction between NEIL1 and WRN (K D = 60 nM) involves C-terminal residues 288-349 of NEIL1 and the RecQ C-terminal (RQC) region of WRN, and is independent of the helicase activity WRN. Exposure to oxidative stress enhances the NEIL-WRN association concomitant with their strong nuclear co-localization. WRN-depleted cells accumulate some prototypical oxidized bases (e.g. 8-oxoguanine, FapyG, and FapyA) indicating a physiological function of WRN in oxidative damage repair in mammalian genomes. Interestingly, WRN deficiency does not have an additive effect on in vivo damage accumulation in NEIL1 knockdown cells suggesting that WRN participates in the same repair pathway as NEIL1.
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U2 - 10.1074/jbc.M703343200
DO - 10.1074/jbc.M703343200
M3 - Article
C2 - 17611195
AN - SCOPUS:34548824343
SN - 0021-9258
VL - 282
SP - 26591
EP - 26602
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 36
ER -