The human DINB1 gene encodes the DNA polymerase Polθ

Robert E. Johnson; Satya Prakash; Louise Prakash

doi:10.1073/pnas.97.8.3838

The human DINB1 gene encodes the DNA polymerase Polθ

Robert E. Johnson, Satya Prakash, Louise Prakash

Biochemistry & Molecular Biology

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159 Scopus citations

Abstract

The human DINB1 gene shares a high degree of homology with the Escherichia coli dinB gene. Here, we purify the hDINB1-encoded protein and show that it is a DNA polymerase. Because hDinB1 is the eighth eukaryotic DNA polymerase to be described, we have named it DNA polymerase (Pol) θ. hPolθ is unable to bypass a cis-syn thymine-thymine dimer, nor does it bypass a (6- 4) photoproduct or an abasic site. We also examine the fidelity of hPolθ on nondamaged DNA templates by steady-state kinetic analyses and find that hPolθ misincorporates deoxynucleotides with a frequency of about 10^-3 to 10^-4. We discuss the relationship between the fidelity of hPolθ and its inability to bypass DNA damage.

Original language	English (US)
Pages (from-to)	3838-3843
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	97
Issue number	8
DOIs	https://doi.org/10.1073/pnas.97.8.3838
State	Published - Apr 11 2000

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abstract = "The human DINB1 gene shares a high degree of homology with the Escherichia coli dinB gene. Here, we purify the hDINB1-encoded protein and show that it is a DNA polymerase. Because hDinB1 is the eighth eukaryotic DNA polymerase to be described, we have named it DNA polymerase (Pol) θ. hPolθ is unable to bypass a cis-syn thymine-thymine dimer, nor does it bypass a (6- 4) photoproduct or an abasic site. We also examine the fidelity of hPolθ on nondamaged DNA templates by steady-state kinetic analyses and find that hPolθ misincorporates deoxynucleotides with a frequency of about 10-3 to 10-4. We discuss the relationship between the fidelity of hPolθ and its inability to bypass DNA damage.",

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The human DINB1 gene encodes the DNA polymerase Polθ

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