@article{c0283623677b4302b16468880bc204ae,
title = "The effects of dorsal rhizotomy and spinal cord isolation on calcitonin gene-related peptide-labeled terminals in the rat lumbar dorsal horn",
abstract = "In the present study, the origin of calcitonin gene-related peptide (CGRP) to the dorsal horn in the rat lumbar spinal cord is investigated. CGRP immunoreactivity is examined following multiple unilateral and bilateral dorsal rhizotomies and isolated cord preparations (spinal cords are isolated by transecting the cord in two places and cutting all dorsal roots between the transections). Seven to 11 days after surgery, unilateral multiple dorsal rhizotomies result in a drastic decrease in CGRP-stained terminals on the operated side; following bilateral dorsal rhizotomies and isolated cord preparations, one or two CGRP varicosities remain in the dorsal horn in each section. The numbers of CGRP-immunostained varicosities observed in the latter two preparations are not significantly different, suggesting that few if any axons descending from the brain contribute to the CGRP terminal population in the spinal cord dorsal horn. Based on these data, we hypothesize that dorsal root ganglion cells are the only source of CGRP to the rat lumbar dorsal horn.",
keywords = "Calcitonin gene-related peptide, Primary afferent, Rat",
author = "K. Chung and Lee, {W. T.} and Carlton, {S. M.}",
note = "Funding Information: We wish to thank Cindy Do for her excellent technical assistance. This study was supported by NIH Grant NSI 1255 (K,C. and S.M.C.) and the Muscular Dystrophy Association (K.C.) 1 Carlton, S.M., McNei!l, D.L. and Coggeshall, R.E., A light and electron microscopic level analysis of calcitonin gene-related peptide (CGRP) in the spinal cord of the primate: an immunohistochemicat study, Neurosci. Lett., 82 (1987) 145-150. 2 Chung, K. and Coggeshall, R,E., Propriospinal fibers in the rat, J. Comp, Neurol., 217 (1983) 47-53. 3 Coggeshall, R.E., Coulter, J.D. and Willis, W.D., Unmyelinated axons in the ventral roots of the cat lumbosacral enlargement, J. Comp. Neurol. 153 (1974) 39-58. 4 Fang, X., The population of the dorsal root ganglion cells which have central processes in ventral rool and their immunoreactivity, Brain Res., 402 (1987) 393-398. 5 Gibson, S.J., Polak, J,M., Bloom, S.R., Sabate, 1.M., Mulderry, P.M., Ghatei, M.A., McGregor, G.P., Morrison, J.F.B., Kelly, J.S., Evans, R.M. and Rosenfeld, M.G., Calcitonin gene-related peptide immunoreactivity in the spinal cord of man and eight other species, J. Neurosci., 4 (1984) 3101 3111. 6 Imai, Y. and Kusama, T., Distribution of the dorsal root fibers in the cat, Brain Res., 13 (1968) 338 359. 7 Ju, G., H6kfelt, T., Brodin, E., Fahrenkrug, J., Fischer, J.A., Frey, P., Elde, R.P. and Brown, J.C, Primary sensory neurons of the rat showing calcitonin gene-related peptide immunoreactivity and their relation to substance P-, galanin-, vasoactive intestinal polypeptide-and cholecystokinin-immunoreac-tive ganglion cells, Cell Tissue Res., 247 (1987) 417-431. 8 Light, A.R. and Metz, C.B., The morphology of the spinal cord efferent and afferent neurons con-tributing to the ventral roots of the cat, J. Comp. Neurol., 179 (1978) 501-516. 9 Light, A.R. and Perl, E.R., Reexamination of the dorsal root projection to the spinal dorsal horn including observations on the differential termination on coarse and fine fibers, J. Comp. Neurol., 186 (1979) 117-132. 10 McNeill, D.L., Chung, K., Carlton, S.M, and Coggeshall, R.E., Calcitonin gene-related peptide immu-nostained axons provide evidence for fine primary afferent fibers in the dorsal and dorsotateral funieuli of rat spinal cord, J. Comp. Neurol., in press, I 1 McNeill, D.L., Cog~geshall, R.E. and Carlton, S.M., A light and electron microscopic study ofcalcito-nin gene-related peptide in the spinal cord of the rat, Exp. Neurol., 93 (1988) 699-708. 12 Rosenfeld, M.G., Mermod, J.J., Amara, S.G., Swanson, L.W., Sawchenko, P.E., Rivier, J., Vale, W.W. and Evans, R.M., Production of a novel neuropeptide encoded by the catcitonin gene via tissue-specific RNA processing, Nature (Lond.) 304 (1983) 129-135. 13 Skofitsch, G. and Jacobowitz, D.M., Calcitonin gene-related peptide coexists with substance P in cap-saicin sensitive neurons and sensory ganglia of the rat, Peptides, 6 (t985) 747-754. 14 Wall, P.D. and Werman, R., The physiology and anatomy of long ranging afferent fibers within the spinal cord, J. Physiol. (Lond.), 255 (1976) 321 334. 15 Yamamoto, T., Takahashi, K., Satomi, H. and lse, H., Origins of primary afferent fibers in the spinal ventral roots in the cat as demonstrated by the horseradish peroxidase method, Brain Res., 126 (1977) 350-354.",
year = "1988",
month = jul,
day = "19",
doi = "10.1016/0304-3940(88)90781-1",
language = "English (US)",
volume = "90",
pages = "27--32",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1-2",
}