The effect of lipid peroxides on isolated human umbilical artery contraction

George R. Saade; Michael A. Belfort; David Johnson; Yuri P. Vedernikov; Helen Hughes; Maya Suresh; Kenneth J. Moise

doi:10.1016/0002-9378(95)90006-3

The effect of lipid peroxides on isolated human umbilical artery contraction

George R. Saade, Michael A. Belfort, David Johnson, Yuri P. Vedernikov, Helen Hughes, Maya Suresh, Kenneth J. Moise

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

OBJECTIVE: Our purpose was to determine the effect of oxidized lipids on the contractile activity of isolated human umbilical arteries. STUDY DESIGN: Umbilical artery rings were prepared for isometric tension recording and exposed to cumulative concentrations of oxidized and nonoxidized lipid and control solutions. Rings were also incubated with the lipid or control solutions and then contracted with cumulative concentrations of U46619. A final set of rings in Ca⁺⁺-free depolarized solution was incubated with the agents above, and then the Ca⁺⁺ concentration was increased cumulatively. RESULTS: The lipids had no direct contractile effect. Both lipids inhibited the response to U46619 and Ca⁺⁺, with the oxidized lipids having the most significant effect. CONCLUSION: Oxidized lipids lack a direct contractile effect on isolated human umbilical arteries and inhibit the contractile response to thromboxane and calcium.

Original language	English (US)
Pages (from-to)	825-830
Number of pages	6
Journal	American journal of obstetrics and gynecology
Volume	172
Issue number	3
DOIs	https://doi.org/10.1016/0002-9378(95)90006-3
State	Published - Mar 1995
Externally published	Yes

Keywords

Human
contraction
in vitro
lipid peroxide
umbilical artery

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1016/0002-9378(95)90006-3

Cite this

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title = "The effect of lipid peroxides on isolated human umbilical artery contraction",

abstract = "OBJECTIVE: Our purpose was to determine the effect of oxidized lipids on the contractile activity of isolated human umbilical arteries. STUDY DESIGN: Umbilical artery rings were prepared for isometric tension recording and exposed to cumulative concentrations of oxidized and nonoxidized lipid and control solutions. Rings were also incubated with the lipid or control solutions and then contracted with cumulative concentrations of U46619. A final set of rings in Ca++-free depolarized solution was incubated with the agents above, and then the Ca++ concentration was increased cumulatively. RESULTS: The lipids had no direct contractile effect. Both lipids inhibited the response to U46619 and Ca++, with the oxidized lipids having the most significant effect. CONCLUSION: Oxidized lipids lack a direct contractile effect on isolated human umbilical arteries and inhibit the contractile response to thromboxane and calcium.",

keywords = "Human, contraction, in vitro, lipid peroxide, umbilical artery",

author = "Saade, {George R.} and Belfort, {Michael A.} and David Johnson and Vedernikov, {Yuri P.} and Helen Hughes and Maya Suresh and Moise, {Kenneth J.}",

year = "1995",

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doi = "10.1016/0002-9378(95)90006-3",

language = "English (US)",

volume = "172",

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TY - JOUR

T1 - The effect of lipid peroxides on isolated human umbilical artery contraction

AU - Saade, George R.

AU - Belfort, Michael A.

AU - Johnson, David

AU - Vedernikov, Yuri P.

AU - Hughes, Helen

AU - Suresh, Maya

AU - Moise, Kenneth J.

PY - 1995/3

Y1 - 1995/3

N2 - OBJECTIVE: Our purpose was to determine the effect of oxidized lipids on the contractile activity of isolated human umbilical arteries. STUDY DESIGN: Umbilical artery rings were prepared for isometric tension recording and exposed to cumulative concentrations of oxidized and nonoxidized lipid and control solutions. Rings were also incubated with the lipid or control solutions and then contracted with cumulative concentrations of U46619. A final set of rings in Ca++-free depolarized solution was incubated with the agents above, and then the Ca++ concentration was increased cumulatively. RESULTS: The lipids had no direct contractile effect. Both lipids inhibited the response to U46619 and Ca++, with the oxidized lipids having the most significant effect. CONCLUSION: Oxidized lipids lack a direct contractile effect on isolated human umbilical arteries and inhibit the contractile response to thromboxane and calcium.

AB - OBJECTIVE: Our purpose was to determine the effect of oxidized lipids on the contractile activity of isolated human umbilical arteries. STUDY DESIGN: Umbilical artery rings were prepared for isometric tension recording and exposed to cumulative concentrations of oxidized and nonoxidized lipid and control solutions. Rings were also incubated with the lipid or control solutions and then contracted with cumulative concentrations of U46619. A final set of rings in Ca++-free depolarized solution was incubated with the agents above, and then the Ca++ concentration was increased cumulatively. RESULTS: The lipids had no direct contractile effect. Both lipids inhibited the response to U46619 and Ca++, with the oxidized lipids having the most significant effect. CONCLUSION: Oxidized lipids lack a direct contractile effect on isolated human umbilical arteries and inhibit the contractile response to thromboxane and calcium.

KW - Human

KW - contraction

KW - in vitro

KW - lipid peroxide

KW - umbilical artery

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ER -

The effect of lipid peroxides on isolated human umbilical artery contraction

Abstract

Keywords

ASJC Scopus subject areas

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