The effect of gastrin on growth of human stomach cancer cells

Jin Ishizuka, Jean Martinez, Courtney M. Townsend, James C. Thompson

Research output: Contribution to journalArticlepeer-review

67 Scopus citations


Gastrin is known as a trophic factor for some stomach and colorectal cancer cells; however, the roles of gastrin receptors and the intracellular signal transduction pathways by which gastrin regulates cell growth are still unknown. The authors examined the effect of synthetic human gastrin-17 on growth of human stomach cancer cells (the parent line, AGS-P, and two different clones, AGS-10 and ACS-12), which were established (and have been maintained) in our laboratory. Gastrin stimulated growth of AGS-P and AGS-10 cells, which have gastrin receptors, in a dose-dependent fashion. A highly selective gastrin receptor antagonist, JMV 320, inhibited the growth-stimulatory effect of gastrin on AGS-P cells in a dose-dependent fashion. Concentrations of gastrin (10-8 to 10-6 M), which stimulated growth of AGS-P cells, did not affect either cyclic adenosine monophosphate production or phosphatidylinositol hydrolysis. Gastrin (10-11 to 10-5 M) mobilized calcium from the intracellular organelles to increase intracellular calcium level in AGS-P cells. The AGS-12 clone has no gastrin receptors, and gastrin did not affect growth or mobilization of intracellular calcium in these cells. Our findings indicate that gastrin stimulates growth of AGS cells through a mechanism that involves binding to specific gastrin receptors that are linked to the system for mobilization of intracellular calcium.

Original languageEnglish (US)
Pages (from-to)528-534
Number of pages7
JournalAnnals of surgery
Issue number5
StatePublished - 1992

ASJC Scopus subject areas

  • Surgery


Dive into the research topics of 'The effect of gastrin on growth of human stomach cancer cells'. Together they form a unique fingerprint.

Cite this