The characterization and crystallization of a virally encoded Ustilago maydis KP4 toxin

Fei Gu; Tiffany S. Sullivan; Zhiwei Che; Chandrashekar Ganesa; William H. Flurkey; Robert F. Bozarth; Thomas J. Smith

doi:10.1016/0022-2836(94)90048-5

The characterization and crystallization of a virally encoded Ustilago maydis KP4 toxin

Fei Gu, Tiffany S. Sullivan, Zhiwei Che, Chandrashekar Ganesa, William H. Flurkey, Robert F. Bozarth, Thomas J. Smith

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

KP4 is a virally encoded and highly specific toxin that kills fungi closely related to the fungus Ustilago maydis. The toxin was purified and crystals were formed using ammonium sulfate as precipitant. The crystals belong to the space group P6₁₍₅₎22 and diffracted to ∼2.2 Å resolution. Circular dicroism spectroscopy suggests that the protein is predominantly comprised of β-strands.

Original language	English (US)
Pages (from-to)	792-795
Number of pages	4
Journal	Journal of Molecular Biology
Volume	243
Issue number	4
DOIs	https://doi.org/10.1016/0022-2836(94)90048-5
State	Published - Nov 4 1994
Externally published	Yes

Keywords

Ustilago maydis
crystallization
fungus
toxin
virus

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/0022-2836(94)90048-5

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title = "The characterization and crystallization of a virally encoded Ustilago maydis KP4 toxin",

abstract = "KP4 is a virally encoded and highly specific toxin that kills fungi closely related to the fungus Ustilago maydis. The toxin was purified and crystals were formed using ammonium sulfate as precipitant. The crystals belong to the space group P61(5)22 and diffracted to ∼2.2 {\AA} resolution. Circular dicroism spectroscopy suggests that the protein is predominantly comprised of β-strands.",

keywords = "Ustilago maydis, crystallization, fungus, toxin, virus",

author = "Fei Gu and Sullivan, {Tiffany S.} and Zhiwei Che and Chandrashekar Ganesa and Flurkey, {William H.} and Bozarth, {Robert F.} and Smith, {Thomas J.}",

note = "Funding Information: work was supported by of Health (GM10704 J? hlarkey Charitable Center).",

year = "1994",

month = nov,

day = "4",

doi = "10.1016/0022-2836(94)90048-5",

language = "English (US)",

volume = "243",

pages = "792--795",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press Inc.",

number = "4",

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T1 - The characterization and crystallization of a virally encoded Ustilago maydis KP4 toxin

AU - Gu, Fei

AU - Sullivan, Tiffany S.

AU - Che, Zhiwei

AU - Ganesa, Chandrashekar

AU - Flurkey, William H.

AU - Bozarth, Robert F.

AU - Smith, Thomas J.

N1 - Funding Information: work was supported by of Health (GM10704 J? hlarkey Charitable Center).

PY - 1994/11/4

Y1 - 1994/11/4

N2 - KP4 is a virally encoded and highly specific toxin that kills fungi closely related to the fungus Ustilago maydis. The toxin was purified and crystals were formed using ammonium sulfate as precipitant. The crystals belong to the space group P61(5)22 and diffracted to ∼2.2 Å resolution. Circular dicroism spectroscopy suggests that the protein is predominantly comprised of β-strands.

AB - KP4 is a virally encoded and highly specific toxin that kills fungi closely related to the fungus Ustilago maydis. The toxin was purified and crystals were formed using ammonium sulfate as precipitant. The crystals belong to the space group P61(5)22 and diffracted to ∼2.2 Å resolution. Circular dicroism spectroscopy suggests that the protein is predominantly comprised of β-strands.

KW - Ustilago maydis

KW - crystallization

KW - fungus

KW - toxin

KW - virus

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DO - 10.1016/0022-2836(94)90048-5

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JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 4

ER -

The characterization and crystallization of a virally encoded Ustilago maydis KP4 toxin

Abstract

Keywords

ASJC Scopus subject areas

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