Temporal global changes in gene expression during temperature transition in Yersinia pestis

Vladimir L. Motin; Anca M. Georgescu; Joseph P. Fitch; Pauline P. Gu; David O. Nelson; Shalini L. Mabery; Janine B. Garnham; Bahrad A. Sokhansanj; Linda L. Ott; Matthew A. Coleman; Jeffrey M. Elliott; Laura M. Kegelmeyer; Andrew J. Wyrobek; Thomas R. Slezak; Robert R. Brubaker; Emilio Garcia

doi:10.1128/JB.186.18.6298-6305.2004

Temporal global changes in gene expression during temperature transition in Yersinia pestis

Vladimir L. Motin, Anca M. Georgescu, Joseph P. Fitch, Pauline P. Gu, David O. Nelson, Shalini L. Mabery, Janine B. Garnham, Bahrad A. Sokhansanj, Linda L. Ott, Matthew A. Coleman, Jeffrey M. Elliott, Laura M. Kegelmeyer, Andrew J. Wyrobek, Thomas R. Slezak, Robert R. Brubaker, Emilio Garcia

Pathology

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

DNA microarrays encompassing the entire genome of Yersinia pestis were used to characterize global regulatory changes during steady-state vegetative growth occurring after shift from 26 to 37°C in the presence and absence of Ca²⁺. Transcriptional profiles revealed that 51, 4, and 13 respective genes and open reading frames (ORFs) on pCD, pPCP, and pMT were thermoinduced and that the majority of these genes carried by pCD were downregulated by Ca²⁺. In contrast, Ca²⁺ had little effect on chromosomal genes and ORFs, of which 235 were thermally upregulated and 274 were thermally downregulated. The primary consequence of these regulatory events is profligate catabolism of numerous metabolites available in the mammalian host.

Original language	English (US)
Pages (from-to)	6298-6305
Number of pages	8
Journal	Journal of bacteriology
Volume	186
Issue number	18
DOIs	https://doi.org/10.1128/JB.186.18.6298-6305.2004
State	Published - Sep 2004

ASJC Scopus subject areas

Microbiology
Molecular Biology

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Motin, V. L., Georgescu, A. M., Fitch, J. P., Gu, P. P., Nelson, D. O., Mabery, S. L., Garnham, J. B., Sokhansanj, B. A., Ott, L. L., Coleman, M. A., Elliott, J. M., Kegelmeyer, L. M., Wyrobek, A. J., Slezak, T. R., Brubaker, R. R., & Garcia, E. (2004). Temporal global changes in gene expression during temperature transition in Yersinia pestis. Journal of bacteriology, 186(18), 6298-6305. https://doi.org/10.1128/JB.186.18.6298-6305.2004

Motin, VL, Georgescu, AM, Fitch, JP, Gu, PP, Nelson, DO, Mabery, SL, Garnham, JB, Sokhansanj, BA, Ott, LL, Coleman, MA, Elliott, JM, Kegelmeyer, LM, Wyrobek, AJ, Slezak, TR, Brubaker, RR & Garcia, E 2004, 'Temporal global changes in gene expression during temperature transition in Yersinia pestis', Journal of bacteriology, vol. 186, no. 18, pp. 6298-6305. https://doi.org/10.1128/JB.186.18.6298-6305.2004

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title = "Temporal global changes in gene expression during temperature transition in Yersinia pestis",

abstract = "DNA microarrays encompassing the entire genome of Yersinia pestis were used to characterize global regulatory changes during steady-state vegetative growth occurring after shift from 26 to 37°C in the presence and absence of Ca2+. Transcriptional profiles revealed that 51, 4, and 13 respective genes and open reading frames (ORFs) on pCD, pPCP, and pMT were thermoinduced and that the majority of these genes carried by pCD were downregulated by Ca2+. In contrast, Ca2+ had little effect on chromosomal genes and ORFs, of which 235 were thermally upregulated and 274 were thermally downregulated. The primary consequence of these regulatory events is profligate catabolism of numerous metabolites available in the mammalian host.",

author = "Motin, {Vladimir L.} and Georgescu, {Anca M.} and Fitch, {Joseph P.} and Gu, {Pauline P.} and Nelson, {David O.} and Mabery, {Shalini L.} and Garnham, {Janine B.} and Sokhansanj, {Bahrad A.} and Ott, {Linda L.} and Coleman, {Matthew A.} and Elliott, {Jeffrey M.} and Kegelmeyer, {Laura M.} and Wyrobek, {Andrew J.} and Slezak, {Thomas R.} and Brubaker, {Robert R.} and Emilio Garcia",

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AU - Motin, Vladimir L.

AU - Georgescu, Anca M.

AU - Fitch, Joseph P.

AU - Gu, Pauline P.

AU - Nelson, David O.

AU - Mabery, Shalini L.

AU - Garnham, Janine B.

AU - Sokhansanj, Bahrad A.

AU - Ott, Linda L.

AU - Coleman, Matthew A.

AU - Elliott, Jeffrey M.

AU - Kegelmeyer, Laura M.

AU - Wyrobek, Andrew J.

AU - Slezak, Thomas R.

AU - Brubaker, Robert R.

AU - Garcia, Emilio

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AB - DNA microarrays encompassing the entire genome of Yersinia pestis were used to characterize global regulatory changes during steady-state vegetative growth occurring after shift from 26 to 37°C in the presence and absence of Ca2+. Transcriptional profiles revealed that 51, 4, and 13 respective genes and open reading frames (ORFs) on pCD, pPCP, and pMT were thermoinduced and that the majority of these genes carried by pCD were downregulated by Ca2+. In contrast, Ca2+ had little effect on chromosomal genes and ORFs, of which 235 were thermally upregulated and 274 were thermally downregulated. The primary consequence of these regulatory events is profligate catabolism of numerous metabolites available in the mammalian host.

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Temporal global changes in gene expression during temperature transition in Yersinia pestis

Abstract

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