Tau oligomers as pathogenic seeds: Preparation and propagation in vitro and in vivo

Julia E. Gerson, Urmi Sengupta, Rakez Kayed

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Scopus citations

Abstract

Tau oligomers have been shown to be the main toxic tau species in a number of neurodegenerative disorders. In order to study tau oligomers both in vitro and in vivo, we have established methods for the reliable preparation, isolation, and detection of tau oligomers. Methods for the seeding of tau oligomers, isolation of tau oligomers from tissue, and detection of tau oligomers using tau oligomer-specific antibodies by biochemical and immunohistochemical methods are detailed below.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages141-157
Number of pages17
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1523
ISSN (Print)1064-3745

Keywords

  • Amyloid seeding
  • Tau oligomer isolation
  • Tau oligomers
  • Tauopathies

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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