Synthesis and utilization of 13C(8)-enriched purines

Christopher J. LaFrancois; June Fujimoto; Lawrence C. Sowers

doi:10.1080/07328319908045591

Synthesis and utilization of ¹³C(8)-enriched purines

Christopher J. LaFrancois, June Fujimoto, Lawrence C. Sowers

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A new and more efficient method is presented for the synthesis of ¹³C(8)-enriched adenine. In addition, we present for the first time the synthesis of ¹³C(8)-enriched 2-aminopurine and purine. All three analogues have been converted to the corresponding ribonucleosides. The adenine analogue has been further converted to the 2'-deoxy-nucleoside and incorporated into a synthetic oligonucleotide. Data is presented demonstrating the utility of ¹³C-enrichment in heteronuclear isotope- edited NMR spectra.

Original language	English (US)
Pages (from-to)	23-37
Number of pages	15
Journal	Nucleosides and Nucleotides
Volume	18
Issue number	1
DOIs	https://doi.org/10.1080/07328319908045591
State	Published - 1999
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Genetics

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10.1080/07328319908045591

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abstract = "A new and more efficient method is presented for the synthesis of 13C(8)-enriched adenine. In addition, we present for the first time the synthesis of 13C(8)-enriched 2-aminopurine and purine. All three analogues have been converted to the corresponding ribonucleosides. The adenine analogue has been further converted to the 2'-deoxy-nucleoside and incorporated into a synthetic oligonucleotide. Data is presented demonstrating the utility of 13C-enrichment in heteronuclear isotope- edited NMR spectra.",

author = "LaFrancois, {Christopher J.} and June Fujimoto and Sowers, {Lawrence C.}",

note = "Funding Information: This work was supported in part by GM41336 and GM50351.",

year = "1999",

doi = "10.1080/07328319908045591",

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T1 - Synthesis and utilization of 13C(8)-enriched purines

AU - LaFrancois, Christopher J.

AU - Fujimoto, June

AU - Sowers, Lawrence C.

N1 - Funding Information: This work was supported in part by GM41336 and GM50351.

PY - 1999

Y1 - 1999

N2 - A new and more efficient method is presented for the synthesis of 13C(8)-enriched adenine. In addition, we present for the first time the synthesis of 13C(8)-enriched 2-aminopurine and purine. All three analogues have been converted to the corresponding ribonucleosides. The adenine analogue has been further converted to the 2'-deoxy-nucleoside and incorporated into a synthetic oligonucleotide. Data is presented demonstrating the utility of 13C-enrichment in heteronuclear isotope- edited NMR spectra.

AB - A new and more efficient method is presented for the synthesis of 13C(8)-enriched adenine. In addition, we present for the first time the synthesis of 13C(8)-enriched 2-aminopurine and purine. All three analogues have been converted to the corresponding ribonucleosides. The adenine analogue has been further converted to the 2'-deoxy-nucleoside and incorporated into a synthetic oligonucleotide. Data is presented demonstrating the utility of 13C-enrichment in heteronuclear isotope- edited NMR spectra.

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Synthesis and utilization of ¹³C(8)-enriched purines

Abstract

ASJC Scopus subject areas

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