1H, 13C and 15N NMR assignments of inactive form of P1 endolysin Lyz

Maruthi Kashyap; Zeenia Jagga; Bhaba Krishna Das; Arulandu Arockiasamy; Neel Sarovar Bhavesh

doi:10.1007/s12104-011-9331-4

¹H, ¹³C and ¹⁵N NMR assignments of inactive form of P1 endolysin Lyz

Maruthi Kashyap, Zeenia Jagga, Bhaba Krishna Das, Arulandu Arockiasamy, Neel Sarovar Bhavesh

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Lysozyme (Lyz) encoded by phage P1 is required for host cell lysis upon infection. Lyz has a N-terminal Signal Anchor Release (SAR) domain, responsible for its secretion into the periplasm and for its accumulation in a membrane tethered inactive form. Here, we report sequence-specific ¹H, ¹³C and ¹⁵N resonance assignments for secreted inactive form of Lyz at pH 4.5.

Original language	English (US)
Pages (from-to)	87-89
Number of pages	3
Journal	Biomolecular NMR Assignments
Volume	6
Issue number	1
DOIs	https://doi.org/10.1007/s12104-011-9331-4
State	Published - Apr 2012
Externally published	Yes

Keywords

Cell lysis
Endolysin
Phage P1

ASJC Scopus subject areas

Structural Biology
Biochemistry

Access to Document

10.1007/s12104-011-9331-4

Cite this

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abstract = "Lysozyme (Lyz) encoded by phage P1 is required for host cell lysis upon infection. Lyz has a N-terminal Signal Anchor Release (SAR) domain, responsible for its secretion into the periplasm and for its accumulation in a membrane tethered inactive form. Here, we report sequence-specific 1H, 13C and 15N resonance assignments for secreted inactive form of Lyz at pH 4.5.",

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AU - Jagga, Zeenia

AU - Das, Bhaba Krishna

AU - Arockiasamy, Arulandu

AU - Bhavesh, Neel Sarovar

PY - 2012/4

Y1 - 2012/4

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AB - Lysozyme (Lyz) encoded by phage P1 is required for host cell lysis upon infection. Lyz has a N-terminal Signal Anchor Release (SAR) domain, responsible for its secretion into the periplasm and for its accumulation in a membrane tethered inactive form. Here, we report sequence-specific 1H, 13C and 15N resonance assignments for secreted inactive form of Lyz at pH 4.5.

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KW - Endolysin

KW - Phage P1

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