TY - JOUR
T1 - Successful refolding and NMR structure of rMagi3
T2 - A disulfide-rich insecticidal spider toxin
AU - Titaux-Delgado, Gustavo
AU - Carrillo, Elisa
AU - Mendoza, Angeles
AU - Mayorga-Flores, Marlen
AU - Escobedo-González, Fátima C.
AU - Cano-Sánchez, Patricia
AU - López-Vera, Estuardo
AU - Corzo, Gerardo
AU - del Rio-Portilla, Federico
N1 - Publisher Copyright:
© 2017 The Protein Society
PY - 2018/3
Y1 - 2018/3
N2 - The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly selective toxins for insect preys. In this respect, spiders as a highly diversified group of almost exclusive insect predators appear to possess infinite potential for the discovery of novel insect-selective toxins. In 2003, a group of toxins was isolated from the spider Macrothele gigas and the amino acid sequence was reported. We obtained, by molecular biology techniques in a heterologous system, one of these toxins. Purification process was optimized by chromatographic methods to determine the three-dimensional structure by nuclear magnetic resonance in solution, and, finally, their biological activity was tested. rMagi3 resulted to be a specific insect toxin with no effect on mice.
AB - The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly selective toxins for insect preys. In this respect, spiders as a highly diversified group of almost exclusive insect predators appear to possess infinite potential for the discovery of novel insect-selective toxins. In 2003, a group of toxins was isolated from the spider Macrothele gigas and the amino acid sequence was reported. We obtained, by molecular biology techniques in a heterologous system, one of these toxins. Purification process was optimized by chromatographic methods to determine the three-dimensional structure by nuclear magnetic resonance in solution, and, finally, their biological activity was tested. rMagi3 resulted to be a specific insect toxin with no effect on mice.
KW - Macrothele gigas
KW - NMR solution structure
KW - insecticidal
KW - refolding disulfide-bonded toxins
KW - spider toxin
UR - http://www.scopus.com/inward/record.url?scp=85039995557&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85039995557&partnerID=8YFLogxK
U2 - 10.1002/pro.3363
DO - 10.1002/pro.3363
M3 - Article
C2 - 29247580
AN - SCOPUS:85039995557
SN - 0961-8368
VL - 27
SP - 692
EP - 701
JO - Protein Science
JF - Protein Science
IS - 3
ER -