TY - JOUR
T1 - Substitution of NS5 N-terminal domain of dengue virus type 2 RNA with type 4 domain caused impaired replication and emergence of adaptive mutants with enhanced fitness
AU - Teramoto, Tadahisa
AU - Boonyasuppayakorn, Siwaporn
AU - Handley, Misty
AU - Choi, Kyung H.
AU - Padmanabhan, Radhakrishnan
PY - 2014/8/8
Y1 - 2014/8/8
N2 - Background: The four dengue virus serotypes, evolved from a common ancestor, are global human pathogens. Results: Interserotypic substitution of the functional domain essential for 5′-capping was detrimental to RNA replication. Conclusion: The chimeric RNA gradually evolves replication fitness through adaptive mutations in genes encoding two replication proteins. Significance: This study provides a possible pathway for generating attenuated dengue virus vaccine.
AB - Background: The four dengue virus serotypes, evolved from a common ancestor, are global human pathogens. Results: Interserotypic substitution of the functional domain essential for 5′-capping was detrimental to RNA replication. Conclusion: The chimeric RNA gradually evolves replication fitness through adaptive mutations in genes encoding two replication proteins. Significance: This study provides a possible pathway for generating attenuated dengue virus vaccine.
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U2 - 10.1074/jbc.M114.584466
DO - 10.1074/jbc.M114.584466
M3 - Article
C2 - 24904061
AN - SCOPUS:84905841271
SN - 0021-9258
VL - 289
SP - 22385
EP - 22400
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 32
ER -