TY - JOUR
T1 - Studies of the pathophysiological mechanisms in frontotemporal dementia by proteome analysis of CSF proteins
AU - Davidsson, Pia
AU - Sjögren, Magnus
AU - Andreasen, Niels
AU - Lindbjer, Maria
AU - Nilsson, Carol L.
AU - Westman-Brinkmalm, Ann
AU - Blennow, Kaj
N1 - Funding Information:
This work was supported by grants from The Swedish Medical Research Council (grants 12769, 13121), Alzheimerfonden, Lund, Sweden; Stiftelsen Gamla Tjännarinnor, Stockholm, Sweden, Stiftelsen Handlanden Hjalmar Svenssons Forskningsfond, Göteborg, Sweden; Wilhelm och Martina Lundgrens Stiftelse, Göteborg, Sweden, Gun och Bertil Stohnes Stiftelse, Kista, Sweden, IngaBritt och Arne Lundbergs Stiftelse, Lund, Sweden, and the Swedish Society of Medicine, Stockholm, Sweden. The authors are also grateful to G. Brinkmalm for help with the data analysis software.
PY - 2002/12/30
Y1 - 2002/12/30
N2 - Comparative proteomic analysis of cerebrospinal fluid (CSF) proteins was employed for studies of the pathophysiological mechanisms in frontotemporal dementia (FTD). Two-dimensional gel electrophoresis and mass spectrometry were used for clinical screening of disease-influenced CSF proteins in 15 FTD patients compared to 12 controls. Six proteins were significantly altered in FTD compared to controls, including granin-like neuroendocrine precursor (proSAAS), pigment-epithelium derived factor (PEDF), retinol-binding protein (RBP), apoE, haptoglobin, and albumin. The levels of ProSAAS, PEDF, and RBP have not been shown earlier to be involved in the FTD pathology. Recently, we have also used proteomic analysis for studies of disease-influenced CSF proteins in Alzheimer's disease (AD) patients. The most clearly affected CSF proteins were the apolipoproteins in AD, compared to controls and FTD patients. ApoE seems to be influenced to a lesser degree in FTD compared to AD. Our data showed that several proteins involved in FTD pathology are not influenced in the CSF of AD patients, and vice versa, establishing differences in the pathophysiological mechanisms between FTD and AD, two of the most common neurodegenerative disorders.
AB - Comparative proteomic analysis of cerebrospinal fluid (CSF) proteins was employed for studies of the pathophysiological mechanisms in frontotemporal dementia (FTD). Two-dimensional gel electrophoresis and mass spectrometry were used for clinical screening of disease-influenced CSF proteins in 15 FTD patients compared to 12 controls. Six proteins were significantly altered in FTD compared to controls, including granin-like neuroendocrine precursor (proSAAS), pigment-epithelium derived factor (PEDF), retinol-binding protein (RBP), apoE, haptoglobin, and albumin. The levels of ProSAAS, PEDF, and RBP have not been shown earlier to be involved in the FTD pathology. Recently, we have also used proteomic analysis for studies of disease-influenced CSF proteins in Alzheimer's disease (AD) patients. The most clearly affected CSF proteins were the apolipoproteins in AD, compared to controls and FTD patients. ApoE seems to be influenced to a lesser degree in FTD compared to AD. Our data showed that several proteins involved in FTD pathology are not influenced in the CSF of AD patients, and vice versa, establishing differences in the pathophysiological mechanisms between FTD and AD, two of the most common neurodegenerative disorders.
KW - Cerebrospinal fluid
KW - Frontotemporal dementia
KW - Granin-like neuroendocrine precursor
KW - Haptoglobin
KW - Pigment epithelium derived factor
KW - Proteomics
KW - Retinol-binding protein
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U2 - 10.1016/S0169-328X(02)00549-1
DO - 10.1016/S0169-328X(02)00549-1
M3 - Article
C2 - 12531522
AN - SCOPUS:0037203346
SN - 0169-328X
VL - 109
SP - 128
EP - 133
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -