Stimulatory Effects of Cholera Toxin on Arachidonic Acid Metabolism in Chinese Hamster Ovary Cells

Cholera toxin (CT) stimulated the release of arachidonic acid (AA) from Chinese hamster ovary cells with no apparent lag period. CT-induced release of [³H]AA or its metabolites was dose dependent during a 4-hr period of toxin exposure with a minimum effective dose of 0.1 ng/ml. CT-induced release of [³H]AA metabolites began within 15 min of toxin addition and became maximal after approximately 5 hr. Neither CT-A subunit nor CT-B subunit alone caused [³H]AA release. Furthermore, [³H]AA release was not caused by addition of dibutyryl cAMP to the culture medium, indicating that the observed effect of CT on arachidonate metabolism appeared to be independent of cAMP. The effect of CT on AA metabolism is proposed as a possible mechanism leading to the synthesis of prostaglandin E and fluid secretion during cholera.