Stimulation of calcitonin secretion in the pig by calcitonin gene-related peptide

C. W. Cooper; M. B. McPherson; P. K. Seitz; G. H. Greeley; S. K. Abbas; D. W. Pickard; A. D. Care

doi:10.1016/0169-6009(91)90036-Y

Stimulation of calcitonin secretion in the pig by calcitonin gene-related peptide

C. W. Cooper, M. B. McPherson, P. K. Seitz, G. H. Greeley, S. K. Abbas, D. W. Pickard, A. D. Care

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Pig thyroid glands were surgically isolated in situ and perfused with autologous blood to which was added known concentrations of calcitonin gene-related peptide (αCGRP). When thyroids were perfused with measured concentrations of CGRP within the range of 0.6-600 nM, the secretion rate of calcitonin (CT) was stimulated while the release of T₃, T₄, and somatostatin remained unchanged. Specific binding of ¹²⁵I-CGRP to pig thyroid plasma membranes was demonstrated, and binding was inhibited by unlabelled CGRP but not by CT or by other peptides unrelated structurally to CGRP. The findings indicate that the pig thyroid gland contains plasma membrane binding sites for CGRP and that CGRP is capable of stimulating the secretion of CT.

Original language	English (US)
Pages (from-to)	73-79
Number of pages	7
Journal	Bone and Mineral
Volume	12
Issue number	2
DOIs	https://doi.org/10.1016/0169-6009(91)90036-Y
State	Published - Feb 1991
Externally published	Yes

Keywords

Calcitonin
Calcitonin gene-related peptide

ASJC Scopus subject areas

Surgery
Biochemistry
Endocrinology

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10.1016/0169-6009(91)90036-Y

Cite this

@article{5793118a55fe49d6ae00554d22ea81c4,

title = "Stimulation of calcitonin secretion in the pig by calcitonin gene-related peptide",

abstract = "Pig thyroid glands were surgically isolated in situ and perfused with autologous blood to which was added known concentrations of calcitonin gene-related peptide (αCGRP). When thyroids were perfused with measured concentrations of CGRP within the range of 0.6-600 nM, the secretion rate of calcitonin (CT) was stimulated while the release of T3, T4, and somatostatin remained unchanged. Specific binding of 125I-CGRP to pig thyroid plasma membranes was demonstrated, and binding was inhibited by unlabelled CGRP but not by CT or by other peptides unrelated structurally to CGRP. The findings indicate that the pig thyroid gland contains plasma membrane binding sites for CGRP and that CGRP is capable of stimulating the secretion of CT.",

keywords = "Calcitonin, Calcitonin gene-related peptide",

author = "Cooper, {C. W.} and McPherson, {M. B.} and Seitz, {P. K.} and Greeley, {G. H.} and Abbas, {S. K.} and Pickard, {D. W.} and Care, {A. D.}",

note = "Funding Information: This work wass upportedb y a US-UK CooperativeR esearchG rant from the National ScienceF oundationP. ortionso f the findingsw erep resentedto the Physiological Societym eetinga t the Univ. of Leeds,M ay 1989(J Physiol 1989;416:5OP) andt o the AmericanS ocietyo f Bonea ndM ineralR esearchm eetingI,n dianapolis, IN, June 1987(J BoneM ineral Res 1987;2(Suplp):l A155).",

year = "1991",

month = feb,

doi = "10.1016/0169-6009(91)90036-Y",

language = "English (US)",

volume = "12",

pages = "73--79",

journal = "Bone and Mineral",

issn = "0169-6009",

publisher = "Elsevier B.V.",

number = "2",

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TY - JOUR

T1 - Stimulation of calcitonin secretion in the pig by calcitonin gene-related peptide

AU - Cooper, C. W.

AU - McPherson, M. B.

AU - Seitz, P. K.

AU - Greeley, G. H.

AU - Abbas, S. K.

AU - Pickard, D. W.

AU - Care, A. D.

N1 - Funding Information: This work wass upportedb y a US-UK CooperativeR esearchG rant from the National ScienceF oundationP. ortionso f the findingsw erep resentedto the Physiological Societym eetinga t the Univ. of Leeds,M ay 1989(J Physiol 1989;416:5OP) andt o the AmericanS ocietyo f Bonea ndM ineralR esearchm eetingI,n dianapolis, IN, June 1987(J BoneM ineral Res 1987;2(Suplp):l A155).

PY - 1991/2

Y1 - 1991/2

N2 - Pig thyroid glands were surgically isolated in situ and perfused with autologous blood to which was added known concentrations of calcitonin gene-related peptide (αCGRP). When thyroids were perfused with measured concentrations of CGRP within the range of 0.6-600 nM, the secretion rate of calcitonin (CT) was stimulated while the release of T3, T4, and somatostatin remained unchanged. Specific binding of 125I-CGRP to pig thyroid plasma membranes was demonstrated, and binding was inhibited by unlabelled CGRP but not by CT or by other peptides unrelated structurally to CGRP. The findings indicate that the pig thyroid gland contains plasma membrane binding sites for CGRP and that CGRP is capable of stimulating the secretion of CT.

AB - Pig thyroid glands were surgically isolated in situ and perfused with autologous blood to which was added known concentrations of calcitonin gene-related peptide (αCGRP). When thyroids were perfused with measured concentrations of CGRP within the range of 0.6-600 nM, the secretion rate of calcitonin (CT) was stimulated while the release of T3, T4, and somatostatin remained unchanged. Specific binding of 125I-CGRP to pig thyroid plasma membranes was demonstrated, and binding was inhibited by unlabelled CGRP but not by CT or by other peptides unrelated structurally to CGRP. The findings indicate that the pig thyroid gland contains plasma membrane binding sites for CGRP and that CGRP is capable of stimulating the secretion of CT.

KW - Calcitonin

KW - Calcitonin gene-related peptide

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JO - Bone and Mineral

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Stimulation of calcitonin secretion in the pig by calcitonin gene-related peptide

Abstract

Keywords

ASJC Scopus subject areas

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