Splanchnic uptake and oxidation of proljne in healthy subjects

L. Castillo, L. Beaumier, Y. M. Yu, V. R. Yjaing

Research output: Contribution to journalArticlepeer-review


To determine uptake of dietary pro by the splanchnic region in healthy humans, we conducted an experiment in 8 adults receiving an L-amino acid diet providing adequate nitrogen and energy intake for 10 days. On day 7, they received an 8h primed constant administration of L-[C]Pro and L-t5,5,2HJPro (3h fast;5fed) by the IV or oral routes, respectively. On day 10 the routes of administration were reversed. Pro fluxes with the [13C] pro tracer were 80±9.4 and 95±13 and 122±18 and 148±25 Itmol.kg'.h' ; (mean +. SD; fast and fed for IV and oral routes respectively); with pHJ pro, fluxes were higher, 118±12 and 131±14 and 165±18 and 166±7 (fast and fed for IV and oral routes respectively). Splanchnic uptake (fraction of tracer; mean .+ SD) was 0.34.+.0.08 and 0.35±0.12 for fast and fed, respectively, for [CJ pro; and 0.28±0.09 and 0.17±0.08 for the [2H2] pro. Pro oxidation was different for the fed and fast state and 13.2±1 and 27.5±3.4 /unol.kg-'.h-' with [13C] pro given IV, similar rates were found with the oral [13C] tracer. [13C] labeling of plasma cit was greater than orn; labeling was higher with oral [13C]pro. The [3H2] pro tracer did not give rise to labeled om or cit. The findings will be discussed in relation to the splanchnic metabolism of urea cycle intermediates.

Original languageEnglish (US)
Pages (from-to)A284
JournalFASEB Journal
Issue number3
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry
  • Biotechnology


Dive into the research topics of 'Splanchnic uptake and oxidation of proljne in healthy subjects'. Together they form a unique fingerprint.

Cite this