Spatial frequency domain tomography of protoporphyrin IX fluorescence in preclinical glioma models

Soren D. Konecky; Chris M. Owen; Tyler Rice; Pablo A. Valdés; Kolbein Kolste; Brian C. Wilson; Frederic Leblond; David W. Roberts; Keith D. Paulsen; Bruce J. Tromberg

doi:10.1117/1.JBO.17.5.056008

Spatial frequency domain tomography of protoporphyrin IX fluorescence in preclinical glioma models

Soren D. Konecky, Chris M. Owen, Tyler Rice, Pablo A. Valdés, Kolbein Kolste, Brian C. Wilson, Frederic Leblond, David W. Roberts, Keith D. Paulsen, Bruce J. Tromberg

Research output: Contribution to journal › Article › peer-review

Abstract

Multifrequency (0 to 0.3 mm-1), multiwavelength (633, 680, 720, 800, and 820 nm) spatial frequency domain imaging (SFDI) of 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) was used to recover absorption, scattering, and fluorescence properties of glioblastoma multiforme spheroids in tissue-simulating phantoms and in vivo in a mouse model. Three-dimensional tomographic reconstructions of the frequency-dependent remitted light localized the depths of the spheroids within 500 μm, and the total amount of PpIX in the reconstructed images was constant to within 30% when spheroid depth was varied. In vivo tumor-to-normal contrast was greater than ∼1.5 in reduced scattering coefficient for all wavelengths and was ∼1.3 for the tissue concentration of deoxyhemoglobin (ctHb). The study demonstrates the feasibility of SFDI for providing enhanced image guidance during surgical resection of brain tumors.

Original language	English (US)
Article number	056008
Journal	Journal of Biomedical Optics
Volume	17
Issue number	5
DOIs	https://doi.org/10.1117/1.JBO.17.5.056008
State	Published - May 2012
Externally published	Yes

Keywords

Fluorescence
Fourier transforms
Tomography

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Biomedical Engineering

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10.1117/1.JBO.17.5.056008

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@article{0ec2bcc2141d4339b3e9fecaffd9d058,

title = "Spatial frequency domain tomography of protoporphyrin IX fluorescence in preclinical glioma models",

abstract = "Multifrequency (0 to 0.3 mm-1), multiwavelength (633, 680, 720, 800, and 820 nm) spatial frequency domain imaging (SFDI) of 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) was used to recover absorption, scattering, and fluorescence properties of glioblastoma multiforme spheroids in tissue-simulating phantoms and in vivo in a mouse model. Three-dimensional tomographic reconstructions of the frequency-dependent remitted light localized the depths of the spheroids within 500 μm, and the total amount of PpIX in the reconstructed images was constant to within 30% when spheroid depth was varied. In vivo tumor-to-normal contrast was greater than ∼1.5 in reduced scattering coefficient for all wavelengths and was ∼1.3 for the tissue concentration of deoxyhemoglobin (ctHb). The study demonstrates the feasibility of SFDI for providing enhanced image guidance during surgical resection of brain tumors.",

keywords = "Fluorescence, Fourier transforms, Tomography",

author = "Konecky, {Soren D.} and Owen, {Chris M.} and Tyler Rice and Vald{\'e}s, {Pablo A.} and Kolbein Kolste and Wilson, {Brian C.} and Frederic Leblond and Roberts, {David W.} and Paulsen, {Keith D.} and Tromberg, {Bruce J.}",

note = "Funding Information: We thank Chung-Ho Sun for preparation of the GBM spheroids, G. Granger for his gift of the ACBT GBM cell line, Yi-hong Zhou for use of the nude mouse glioma model, and Abhishek Chaturbedi for preparation of this model. Support for this work was provided to University of California–Irvine by the National Institutes of Health (NIH) NCRR/NIBIB Laser Microbeam and Medical Program (LAMMP, P41-EB015890), the Military Photomedicine Program (AFOSR Grant # FA9550-08-1-0384), and the Beckman Foundation. Support to Dartmouth College/University of Toronto was provided through NIH grants RO1-NS052274-01A2 and K25-CA138578. Soren D. Konecky was supported by a fellowship from the Hewitt Foundation for Medical Research.",

year = "2012",

month = may,

doi = "10.1117/1.JBO.17.5.056008",

language = "English (US)",

volume = "17",

journal = "Journal of Biomedical Optics",

issn = "1083-3668",

publisher = "SPIE",

number = "5",

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TY - JOUR

T1 - Spatial frequency domain tomography of protoporphyrin IX fluorescence in preclinical glioma models

AU - Konecky, Soren D.

AU - Owen, Chris M.

AU - Rice, Tyler

AU - Valdés, Pablo A.

AU - Kolste, Kolbein

AU - Wilson, Brian C.

AU - Leblond, Frederic

AU - Roberts, David W.

AU - Paulsen, Keith D.

AU - Tromberg, Bruce J.

N1 - Funding Information: We thank Chung-Ho Sun for preparation of the GBM spheroids, G. Granger for his gift of the ACBT GBM cell line, Yi-hong Zhou for use of the nude mouse glioma model, and Abhishek Chaturbedi for preparation of this model. Support for this work was provided to University of California–Irvine by the National Institutes of Health (NIH) NCRR/NIBIB Laser Microbeam and Medical Program (LAMMP, P41-EB015890), the Military Photomedicine Program (AFOSR Grant # FA9550-08-1-0384), and the Beckman Foundation. Support to Dartmouth College/University of Toronto was provided through NIH grants RO1-NS052274-01A2 and K25-CA138578. Soren D. Konecky was supported by a fellowship from the Hewitt Foundation for Medical Research.

PY - 2012/5

Y1 - 2012/5

N2 - Multifrequency (0 to 0.3 mm-1), multiwavelength (633, 680, 720, 800, and 820 nm) spatial frequency domain imaging (SFDI) of 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) was used to recover absorption, scattering, and fluorescence properties of glioblastoma multiforme spheroids in tissue-simulating phantoms and in vivo in a mouse model. Three-dimensional tomographic reconstructions of the frequency-dependent remitted light localized the depths of the spheroids within 500 μm, and the total amount of PpIX in the reconstructed images was constant to within 30% when spheroid depth was varied. In vivo tumor-to-normal contrast was greater than ∼1.5 in reduced scattering coefficient for all wavelengths and was ∼1.3 for the tissue concentration of deoxyhemoglobin (ctHb). The study demonstrates the feasibility of SFDI for providing enhanced image guidance during surgical resection of brain tumors.

AB - Multifrequency (0 to 0.3 mm-1), multiwavelength (633, 680, 720, 800, and 820 nm) spatial frequency domain imaging (SFDI) of 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) was used to recover absorption, scattering, and fluorescence properties of glioblastoma multiforme spheroids in tissue-simulating phantoms and in vivo in a mouse model. Three-dimensional tomographic reconstructions of the frequency-dependent remitted light localized the depths of the spheroids within 500 μm, and the total amount of PpIX in the reconstructed images was constant to within 30% when spheroid depth was varied. In vivo tumor-to-normal contrast was greater than ∼1.5 in reduced scattering coefficient for all wavelengths and was ∼1.3 for the tissue concentration of deoxyhemoglobin (ctHb). The study demonstrates the feasibility of SFDI for providing enhanced image guidance during surgical resection of brain tumors.

KW - Fluorescence

KW - Fourier transforms

KW - Tomography

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AN - SCOPUS:84866790122

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JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

IS - 5

M1 - 056008

ER -

Spatial frequency domain tomography of protoporphyrin IX fluorescence in preclinical glioma models

Abstract

Keywords

ASJC Scopus subject areas

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