Spatial frequency domain tomography of protoporphyrin IX fluorescence in preclinical glioma models

Soren D. Konecky, Chris M. Owen, Tyler Rice, Pablo A. Valdés, Kolbein Kolste, Brian C. Wilson, Frederic Leblond, David W. Roberts, Keith D. Paulsen, Bruce J. Tromberg

Research output: Contribution to journalArticlepeer-review


Multifrequency (0 to 0.3 mm-1), multiwavelength (633, 680, 720, 800, and 820 nm) spatial frequency domain imaging (SFDI) of 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) was used to recover absorption, scattering, and fluorescence properties of glioblastoma multiforme spheroids in tissue-simulating phantoms and in vivo in a mouse model. Three-dimensional tomographic reconstructions of the frequency-dependent remitted light localized the depths of the spheroids within 500 μm, and the total amount of PpIX in the reconstructed images was constant to within 30% when spheroid depth was varied. In vivo tumor-to-normal contrast was greater than ∼1.5 in reduced scattering coefficient for all wavelengths and was ∼1.3 for the tissue concentration of deoxyhemoglobin (ctHb). The study demonstrates the feasibility of SFDI for providing enhanced image guidance during surgical resection of brain tumors.

Original languageEnglish (US)
Article number056008
JournalJournal of Biomedical Optics
Issue number5
StatePublished - May 2012
Externally publishedYes


  • Fluorescence
  • Fourier transforms
  • Tomography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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