Soluble IL-4 receptor improves the skin-graft-associated cytomegalovirus infection in thermally injured mice

Hiroyuki Kobayashi; Makiko Kobayashi; Hitoshi Takahashi; David N. Herndon; Richard B. Pollard; Fujio Suzuki

doi:10.1016/S0305-4179(02)00200-0

Soluble IL-4 receptor improves the skin-graft-associated cytomegalovirus infection in thermally injured mice

Hiroyuki Kobayashi, Makiko Kobayashi, Hitoshi Takahashi, David N. Herndon, Richard B. Pollard, Fujio Suzuki

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The susceptibility of thermally injured mice (TI-mice) to murine cytomegalovirus (MCMV) infection is markedly influenced by burn-associated type 2 T cell responses, which are common with severe thermal injuries. In the present study, the effect of soluble IL-4 receptor (sIL-4R) on the skin-graft-associated MCMV infection was investigated. The marked growth of MCMV was demonstrated in the salivary glands of TI-mice grafted with MCMV seropositive [MCMV sero(+)] skin. However, the growth of MCMV was not demonstrated in the salivary glands of TI-mice grafted with MCMV sero(+) skin and treated with 50ng per mouse of sIL-4R. Compared with grafted normal mice, production of type 1 cytokines was markedly decreased when splenic T cells from TI-mice grafted with MCMV sero(+) skin were stimulated with anti-CD3 monoclonal antibody (mAb). The impaired type 1 cytokine production was recovered in cultures of splenic T cells from grafted TI-mice previously treated with sIL-4R. After grafting with MCMV sero(+) skin, the growth of MCMV was markedly inhibited in the salivary glands of severe combined immunodeficient (SCID) mice inoculated with T cells from TI-mice treated with sIL-4R. These results suggest that sIL-4R regulates the skin-graft-associated MCMV infection in TI-mice.

Original language	English (US)
Pages (from-to)	315-321
Number of pages	7
Journal	Burns
Volume	29
Issue number	4
DOIs	https://doi.org/10.1016/S0305-4179(02)00200-0
State	Published - Jun 2003

Keywords

Burn injury
Cytomegalovirus
Soluble IL-4 receptor

ASJC Scopus subject areas

Surgery
Emergency Medicine
Critical Care and Intensive Care Medicine

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10.1016/S0305-4179(02)00200-0

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@article{7cf231f2073648c996460041a0a9d8e4,

title = "Soluble IL-4 receptor improves the skin-graft-associated cytomegalovirus infection in thermally injured mice",

abstract = "The susceptibility of thermally injured mice (TI-mice) to murine cytomegalovirus (MCMV) infection is markedly influenced by burn-associated type 2 T cell responses, which are common with severe thermal injuries. In the present study, the effect of soluble IL-4 receptor (sIL-4R) on the skin-graft-associated MCMV infection was investigated. The marked growth of MCMV was demonstrated in the salivary glands of TI-mice grafted with MCMV seropositive [MCMV sero(+)] skin. However, the growth of MCMV was not demonstrated in the salivary glands of TI-mice grafted with MCMV sero(+) skin and treated with 50ng per mouse of sIL-4R. Compared with grafted normal mice, production of type 1 cytokines was markedly decreased when splenic T cells from TI-mice grafted with MCMV sero(+) skin were stimulated with anti-CD3 monoclonal antibody (mAb). The impaired type 1 cytokine production was recovered in cultures of splenic T cells from grafted TI-mice previously treated with sIL-4R. After grafting with MCMV sero(+) skin, the growth of MCMV was markedly inhibited in the salivary glands of severe combined immunodeficient (SCID) mice inoculated with T cells from TI-mice treated with sIL-4R. These results suggest that sIL-4R regulates the skin-graft-associated MCMV infection in TI-mice.",

keywords = "Burn injury, Cytomegalovirus, Soluble IL-4 receptor",

author = "Hiroyuki Kobayashi and Makiko Kobayashi and Hitoshi Takahashi and Herndon, {David N.} and Pollard, {Richard B.} and Fujio Suzuki",

year = "2003",

month = jun,

doi = "10.1016/S0305-4179(02)00200-0",

language = "English (US)",

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pages = "315--321",

journal = "Burns",

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number = "4",

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T1 - Soluble IL-4 receptor improves the skin-graft-associated cytomegalovirus infection in thermally injured mice

AU - Kobayashi, Hiroyuki

AU - Kobayashi, Makiko

AU - Takahashi, Hitoshi

AU - Herndon, David N.

AU - Pollard, Richard B.

AU - Suzuki, Fujio

PY - 2003/6

Y1 - 2003/6

N2 - The susceptibility of thermally injured mice (TI-mice) to murine cytomegalovirus (MCMV) infection is markedly influenced by burn-associated type 2 T cell responses, which are common with severe thermal injuries. In the present study, the effect of soluble IL-4 receptor (sIL-4R) on the skin-graft-associated MCMV infection was investigated. The marked growth of MCMV was demonstrated in the salivary glands of TI-mice grafted with MCMV seropositive [MCMV sero(+)] skin. However, the growth of MCMV was not demonstrated in the salivary glands of TI-mice grafted with MCMV sero(+) skin and treated with 50ng per mouse of sIL-4R. Compared with grafted normal mice, production of type 1 cytokines was markedly decreased when splenic T cells from TI-mice grafted with MCMV sero(+) skin were stimulated with anti-CD3 monoclonal antibody (mAb). The impaired type 1 cytokine production was recovered in cultures of splenic T cells from grafted TI-mice previously treated with sIL-4R. After grafting with MCMV sero(+) skin, the growth of MCMV was markedly inhibited in the salivary glands of severe combined immunodeficient (SCID) mice inoculated with T cells from TI-mice treated with sIL-4R. These results suggest that sIL-4R regulates the skin-graft-associated MCMV infection in TI-mice.

AB - The susceptibility of thermally injured mice (TI-mice) to murine cytomegalovirus (MCMV) infection is markedly influenced by burn-associated type 2 T cell responses, which are common with severe thermal injuries. In the present study, the effect of soluble IL-4 receptor (sIL-4R) on the skin-graft-associated MCMV infection was investigated. The marked growth of MCMV was demonstrated in the salivary glands of TI-mice grafted with MCMV seropositive [MCMV sero(+)] skin. However, the growth of MCMV was not demonstrated in the salivary glands of TI-mice grafted with MCMV sero(+) skin and treated with 50ng per mouse of sIL-4R. Compared with grafted normal mice, production of type 1 cytokines was markedly decreased when splenic T cells from TI-mice grafted with MCMV sero(+) skin were stimulated with anti-CD3 monoclonal antibody (mAb). The impaired type 1 cytokine production was recovered in cultures of splenic T cells from grafted TI-mice previously treated with sIL-4R. After grafting with MCMV sero(+) skin, the growth of MCMV was markedly inhibited in the salivary glands of severe combined immunodeficient (SCID) mice inoculated with T cells from TI-mice treated with sIL-4R. These results suggest that sIL-4R regulates the skin-graft-associated MCMV infection in TI-mice.

KW - Burn injury

KW - Cytomegalovirus

KW - Soluble IL-4 receptor

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Soluble IL-4 receptor improves the skin-graft-associated cytomegalovirus infection in thermally injured mice

Abstract

Keywords

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