TY - JOUR
T1 - Single-particle cryo-electron microscopy of Rift Valley fever virus
AU - Sherman, Michael B.
AU - Freiberg, Alexander N.
AU - Holbrook, Michael R.
AU - Watowich, Stanley J.
N1 - Funding Information:
We thank Drs. C.J. Peters and Shinji Makino for helpful discussions. This work was supported by a training fellowship from the W. M. Keck Foundation to the Gulf Coast Consortia through the Keck Center for Virus Imaging (A.N.F.), and by grants from the NIH/NIAID Western Regional Center of Excellence for Biodefense and Emerging Infectious Disease Research (sub-award from U54 AI057156 to M.B.S.) and the Welch Foundation (S.J.W.).
PY - 2009/4/25
Y1 - 2009/4/25
N2 - Rift Valley fever virus (RVFV; Bunyaviridae; Phlebovirus) is an emerging human and veterinary pathogen causing acute hepatitis in ruminants and has the potential to cause hemorrhagic fever in humans. We report a three-dimensional reconstruction of RVFV vaccine strain MP-12 (RVFV MP-12) by cryo-electron microcopy using icosahedral symmetry of individual virions. Although the genomic core of RVFV MP-12 is apparently poorly ordered, the glycoproteins on the virus surface are highly symmetric and arranged on a T = 12 icosahedral lattice. Our RVFV MP-12 structure allowed clear identification of inter-capsomer contacts and definition of possible glycoprotein arrangements within capsomers. This structure provides a detailed model for phleboviruses, opens new avenues for high-resolution structural studies of the bunyavirus family, and aids the design of antiviral diagnostics and effective subunit vaccines.
AB - Rift Valley fever virus (RVFV; Bunyaviridae; Phlebovirus) is an emerging human and veterinary pathogen causing acute hepatitis in ruminants and has the potential to cause hemorrhagic fever in humans. We report a three-dimensional reconstruction of RVFV vaccine strain MP-12 (RVFV MP-12) by cryo-electron microcopy using icosahedral symmetry of individual virions. Although the genomic core of RVFV MP-12 is apparently poorly ordered, the glycoproteins on the virus surface are highly symmetric and arranged on a T = 12 icosahedral lattice. Our RVFV MP-12 structure allowed clear identification of inter-capsomer contacts and definition of possible glycoprotein arrangements within capsomers. This structure provides a detailed model for phleboviruses, opens new avenues for high-resolution structural studies of the bunyavirus family, and aids the design of antiviral diagnostics and effective subunit vaccines.
KW - Bunyaviridae
KW - Cryo-electron microscopy
KW - Inter-capsomer contacts
KW - Rift Valley fever virus
KW - Single-particle averaging
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U2 - 10.1016/j.virol.2009.02.038
DO - 10.1016/j.virol.2009.02.038
M3 - Article
C2 - 19304307
AN - SCOPUS:63549107008
SN - 0042-6822
VL - 387
SP - 11
EP - 15
JO - Virology
JF - Virology
IS - 1
ER -