Simplified microneutralization test for serotyping adenovirus isolates

M. D. Malasig; P. R. Goswami; L. K. Crawford-Miksza; D. P. Schnurr; G. C. Gray

doi:10.1128/JCM.39.8.2984-2986.2001

Simplified microneutralization test for serotyping adenovirus isolates

M. D. Malasig, P. R. Goswami, L. K. Crawford-Miksza, D. P. Schnurr, G. C. Gray

Research output: Contribution to journal › Article › peer-review

Abstract

A simplified microneutralization procedure is described that uses an empirically determined virus challenge dose, a single dilution of antiserum, and observation of cytopathic effect to determine the adenovirus serotype. The simplified test has faster turnaround time and was 96% concordant with a confirmatory test using serial dilutions of type-specific sera. This method will find utility in high-volume serotyping work.

Original language	English (US)
Pages (from-to)	2984-2986
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	39
Issue number	8
DOIs	https://doi.org/10.1128/JCM.39.8.2984-2986.2001
State	Published - 2001
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.39.8.2984-2986.2001

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AU - Goswami, P. R.

AU - Crawford-Miksza, L. K.

AU - Schnurr, D. P.

AU - Gray, G. C.

PY - 2001

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AB - A simplified microneutralization procedure is described that uses an empirically determined virus challenge dose, a single dilution of antiserum, and observation of cytopathic effect to determine the adenovirus serotype. The simplified test has faster turnaround time and was 96% concordant with a confirmatory test using serial dilutions of type-specific sera. This method will find utility in high-volume serotyping work.

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Simplified microneutralization test for serotyping adenovirus isolates

Abstract

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