@article{99ce2eb5238242849b80112805c56e27,
title = "Sequential resonance assignments as a basis for determination of spatial protein structures by high resolution proton nuclear magnetic resonance",
abstract = "A general scheme is proposed for the determination of spatial protein structures by proton nuclear magnetic resonance. The scheme relies on experimental observation by two-dimensional nuclear magnetic resonance techniques of complete throughbond and through-space proton-proton connectivity maps. These are used to obtain sequential resonance assignments for the individual residues in the amino acid sequence and to characterize the spatial polypeptide structure by a tight network of semi-quantitative, intramolecular distance constraints.",
author = "Kurt W{\"u}thrich and Gerhard Wider and Gerhard Wagner and Werner Braun",
note = "Funding Information: The development of two-dimensional n.m.r. techniques for studies of biopolymers. which havtx a crucial role in the realization of the presently outlined scheme for protein structure dt%ermination, is a joint project with Professor R. R. Ernst and is financed by a special grant of the Eidgenijssische Technische Hochschule. Ziirirh. We thank Professor Ernst for continued advice on all aspects of two-dimensional n.m.r. Financial support by the Schweizerischrr Nationalfonds (project 3528.79) is grat,efully acknowledged.",
year = "1982",
month = mar,
day = "5",
doi = "10.1016/0022-2836(82)90007-9",
language = "English (US)",
volume = "155",
pages = "311--319",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "3",
}