Sequence analysis of the 190-kDa antigen-encoding gene of Rickettsia conorii (Malish 7 strain)

Patricia A. Crocquet-Valdes; Kimberly Weiss; D. H. Walker

doi:10.1016/0378-1119(94)90740-4

Sequence analysis of the 190-kDa antigen-encoding gene of Rickettsia conorii (Malish 7 strain)

Patricia A. Crocquet-Valdes, Kimberly Weiss, D. H. Walker

Pathology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The complete nucleotide (nt) sequence of the gene (rompA) encoding the 190-kDa immunodominant surface antigen (rOmpA) of Rickettsia conorii (Malish 7 strain) was determined. Sequence analysis revealed an ORF of 6063 nt encoding a deduced protein of 203 247 Da. Ten consecutive highly conserved repeat units, located at the 5' end of rompA, spanned 2.2 kb. Two types of repeats could be identified: type I of 225 bp, and type II of 216 bp. The order and number of repeats differed from those reported for R. conorii (Kenya tick typhus strain), R. akari (Kaplan strain) and R. rickettsii (R strain). Alignment of the R. conorii (Malish 7 strain) rompA gene with its R. rickettsii homolog revealed 95% nt sequence similarity. The conservation of rompA across several pathogenic spotted fever group (SFG) rickettsial species suggests that it may be a potential candidate for use as a subunit vaccine for SFG rickettsial diseases.

Original language	English (US)
Pages (from-to)	115-119
Number of pages	5
Journal	Gene
Volume	140
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(94)90740-4
State	Published - 1994

Keywords

Recombinant DNA
bacterial antigen
promoter
repetitive sequence
subunit vaccine
terminator

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(94)90740-4

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title = "Sequence analysis of the 190-kDa antigen-encoding gene of Rickettsia conorii (Malish 7 strain)",

abstract = "The complete nucleotide (nt) sequence of the gene (rompA) encoding the 190-kDa immunodominant surface antigen (rOmpA) of Rickettsia conorii (Malish 7 strain) was determined. Sequence analysis revealed an ORF of 6063 nt encoding a deduced protein of 203 247 Da. Ten consecutive highly conserved repeat units, located at the 5' end of rompA, spanned 2.2 kb. Two types of repeats could be identified: type I of 225 bp, and type II of 216 bp. The order and number of repeats differed from those reported for R. conorii (Kenya tick typhus strain), R. akari (Kaplan strain) and R. rickettsii (R strain). Alignment of the R. conorii (Malish 7 strain) rompA gene with its R. rickettsii homolog revealed 95% nt sequence similarity. The conservation of rompA across several pathogenic spotted fever group (SFG) rickettsial species suggests that it may be a potential candidate for use as a subunit vaccine for SFG rickettsial diseases.",

keywords = "Recombinant DNA, bacterial antigen, promoter, repetitive sequence, subunit vaccine, terminator",

author = "Crocquet-Valdes, {Patricia A.} and Kimberly Weiss and Walker, {D. H.}",

year = "1994",

doi = "10.1016/0378-1119(94)90740-4",

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AU - Crocquet-Valdes, Patricia A.

AU - Weiss, Kimberly

AU - Walker, D. H.

PY - 1994

Y1 - 1994

N2 - The complete nucleotide (nt) sequence of the gene (rompA) encoding the 190-kDa immunodominant surface antigen (rOmpA) of Rickettsia conorii (Malish 7 strain) was determined. Sequence analysis revealed an ORF of 6063 nt encoding a deduced protein of 203 247 Da. Ten consecutive highly conserved repeat units, located at the 5' end of rompA, spanned 2.2 kb. Two types of repeats could be identified: type I of 225 bp, and type II of 216 bp. The order and number of repeats differed from those reported for R. conorii (Kenya tick typhus strain), R. akari (Kaplan strain) and R. rickettsii (R strain). Alignment of the R. conorii (Malish 7 strain) rompA gene with its R. rickettsii homolog revealed 95% nt sequence similarity. The conservation of rompA across several pathogenic spotted fever group (SFG) rickettsial species suggests that it may be a potential candidate for use as a subunit vaccine for SFG rickettsial diseases.

AB - The complete nucleotide (nt) sequence of the gene (rompA) encoding the 190-kDa immunodominant surface antigen (rOmpA) of Rickettsia conorii (Malish 7 strain) was determined. Sequence analysis revealed an ORF of 6063 nt encoding a deduced protein of 203 247 Da. Ten consecutive highly conserved repeat units, located at the 5' end of rompA, spanned 2.2 kb. Two types of repeats could be identified: type I of 225 bp, and type II of 216 bp. The order and number of repeats differed from those reported for R. conorii (Kenya tick typhus strain), R. akari (Kaplan strain) and R. rickettsii (R strain). Alignment of the R. conorii (Malish 7 strain) rompA gene with its R. rickettsii homolog revealed 95% nt sequence similarity. The conservation of rompA across several pathogenic spotted fever group (SFG) rickettsial species suggests that it may be a potential candidate for use as a subunit vaccine for SFG rickettsial diseases.

KW - Recombinant DNA

KW - bacterial antigen

KW - promoter

KW - repetitive sequence

KW - subunit vaccine

KW - terminator

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Sequence analysis of the 190-kDa antigen-encoding gene of Rickettsia conorii (Malish 7 strain)

Abstract

Keywords

ASJC Scopus subject areas

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