Sequence analysis of SOD gene of Bombyx mori nuclear polyhedrosis virus and it's expression in E. coli

Wen Bing Wang; Ping Ji; Jun Wu; Jia Lu He; Xiang Fu Wu

Sequence analysis of SOD gene of Bombyx mori nuclear polyhedrosis virus and it's expression in E. coli

Wen Bing Wang, Ping Ji, Jun Wu, Jia Lu He, Xiang Fu Wu

Research output: Contribution to journal › Article › peer-review

Abstract

Superoxide dismutases scavenge superoxide radicals and protect cells from oxidative stress. SOD gene of Bombyx mori nuclear polyhedrosis virus has been cloned by PCR, and is expressed in E. coli with the activity of SOD being 576. 13 u/mL. DNA sequence analysis shows that SOD gene of BmNPV encodes 151 amino acids. The homology between BmNPV and AcNPV of the nucleotide sequences of SOD gene is 97.2%, and 56% between BmNPV and human SOD1.

Original language	English (US)
Pages (from-to)	407-408
Number of pages	2
Journal	Acta Biochimica et Biophysica Sinica
Volume	31
Issue number	4
State	Published - 1999
Externally published	Yes

Keywords

BmNPV
Expression
Sequence analysis
Superoxide dismutase

ASJC Scopus subject areas

Biophysics
Biochemistry

Cite this

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title = "Sequence analysis of SOD gene of Bombyx mori nuclear polyhedrosis virus and it's expression in E. coli",

abstract = "Superoxide dismutases scavenge superoxide radicals and protect cells from oxidative stress. SOD gene of Bombyx mori nuclear polyhedrosis virus has been cloned by PCR, and is expressed in E. coli with the activity of SOD being 576. 13 u/mL. DNA sequence analysis shows that SOD gene of BmNPV encodes 151 amino acids. The homology between BmNPV and AcNPV of the nucleotide sequences of SOD gene is 97.2%, and 56% between BmNPV and human SOD1.",

keywords = "BmNPV, Expression, Sequence analysis, Superoxide dismutase",

author = "Wang, {Wen Bing} and Ping Ji and Jun Wu and He, {Jia Lu} and Wu, {Xiang Fu}",

year = "1999",

language = "English (US)",

volume = "31",

pages = "407--408",

journal = "Acta Biochimica et Biophysica Sinica",

issn = "0582-9879",

publisher = "Shanghai Kexue Jishu Chubanshe/Shanghai Scientific & Technical Publishers",

number = "4",

}

TY - JOUR

T1 - Sequence analysis of SOD gene of Bombyx mori nuclear polyhedrosis virus and it's expression in E. coli

AU - Wang, Wen Bing

AU - Ji, Ping

AU - Wu, Jun

AU - He, Jia Lu

AU - Wu, Xiang Fu

PY - 1999

Y1 - 1999

N2 - Superoxide dismutases scavenge superoxide radicals and protect cells from oxidative stress. SOD gene of Bombyx mori nuclear polyhedrosis virus has been cloned by PCR, and is expressed in E. coli with the activity of SOD being 576. 13 u/mL. DNA sequence analysis shows that SOD gene of BmNPV encodes 151 amino acids. The homology between BmNPV and AcNPV of the nucleotide sequences of SOD gene is 97.2%, and 56% between BmNPV and human SOD1.

AB - Superoxide dismutases scavenge superoxide radicals and protect cells from oxidative stress. SOD gene of Bombyx mori nuclear polyhedrosis virus has been cloned by PCR, and is expressed in E. coli with the activity of SOD being 576. 13 u/mL. DNA sequence analysis shows that SOD gene of BmNPV encodes 151 amino acids. The homology between BmNPV and AcNPV of the nucleotide sequences of SOD gene is 97.2%, and 56% between BmNPV and human SOD1.

KW - BmNPV

KW - Expression

KW - Sequence analysis

KW - Superoxide dismutase

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M3 - Article

AN - SCOPUS:20444376956

SN - 0582-9879

VL - 31

SP - 407

EP - 408

JO - Acta Biochimica et Biophysica Sinica

JF - Acta Biochimica et Biophysica Sinica

IS - 4

ER -

Sequence analysis of SOD gene of Bombyx mori nuclear polyhedrosis virus and it's expression in E. coli

Abstract

Keywords

ASJC Scopus subject areas

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