Sendai virosomal infusion of an adeno-associated virus-derived construct containing neuropeptide Y into primary rat brain cultures

Ping Wu; Christopher M. de Fiebre; William J. Millard; Kristin Elmstrom; Yuping Gao; Edwin M. Meyer

doi:10.1016/0304-3940(95)11503-O

Sendai virosomal infusion of an adeno-associated virus-derived construct containing neuropeptide Y into primary rat brain cultures

Ping Wu, Christopher M. de Fiebre, William J. Millard, Kristin Elmstrom, Yuping Gao, Edwin M. Meyer

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A novel neuronal gene-delivery system was investigated in primary neuron-enriched cultures with respect to driving the expression of neuropeptide Y (NPY). This delivery system consists of an adeno-associated virus-derived (AAV) plasmid, pJDT95npy, encapsulated in reconstituted Sendai virosomes. pJDT95npy contains full length rat NPY cDNA inserted downstream from the P40 promoter in a cap-gene deleted AAV derived construct. The rep-sequences under control of the P5 and P19 promoters are intact. Virosomally encapsulated pJDT95npy drove the expression of NPY mRNAs, predominantly by P40. Total cellular NPY immunoreactivity and release in the presence of depolarization increased following pJDT95npy-transfection. Neither empty virosomes nor virosomes containing pJDT95 affected NPY mRNA expression or immunoreactivity. This study demonstrates that an AAVderived plasmid can drive exogenous gene expression in intact neurons after infusion by Sendai virosomes.

Original language	English (US)
Pages (from-to)	73-76
Number of pages	4
Journal	Neuroscience Letters
Volume	190
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(95)11503-O
State	Published - May 5 1995
Externally published	Yes

Keywords

Adeno-associated virus construct
Neuropeptide Y
Sendai virus

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(95)11503-O

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title = "Sendai virosomal infusion of an adeno-associated virus-derived construct containing neuropeptide Y into primary rat brain cultures",

abstract = "A novel neuronal gene-delivery system was investigated in primary neuron-enriched cultures with respect to driving the expression of neuropeptide Y (NPY). This delivery system consists of an adeno-associated virus-derived (AAV) plasmid, pJDT95npy, encapsulated in reconstituted Sendai virosomes. pJDT95npy contains full length rat NPY cDNA inserted downstream from the P40 promoter in a cap-gene deleted AAV derived construct. The rep-sequences under control of the P5 and P19 promoters are intact. Virosomally encapsulated pJDT95npy drove the expression of NPY mRNAs, predominantly by P40. Total cellular NPY immunoreactivity and release in the presence of depolarization increased following pJDT95npy-transfection. Neither empty virosomes nor virosomes containing pJDT95 affected NPY mRNA expression or immunoreactivity. This study demonstrates that an AAVderived plasmid can drive exogenous gene expression in intact neurons after infusion by Sendai virosomes.",

keywords = "Adeno-associated virus construct, Neuropeptide Y, Sendai virus",

author = "Ping Wu and {de Fiebre}, {Christopher M.} and Millard, {William J.} and Kristin Elmstrom and Yuping Gao and Meyer, {Edwin M.}",

year = "1995",

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doi = "10.1016/0304-3940(95)11503-O",

language = "English (US)",

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pages = "73--76",

journal = "Neuroscience Letters",

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T1 - Sendai virosomal infusion of an adeno-associated virus-derived construct containing neuropeptide Y into primary rat brain cultures

AU - Wu, Ping

AU - de Fiebre, Christopher M.

AU - Millard, William J.

AU - Elmstrom, Kristin

AU - Gao, Yuping

AU - Meyer, Edwin M.

PY - 1995/5/5

Y1 - 1995/5/5

N2 - A novel neuronal gene-delivery system was investigated in primary neuron-enriched cultures with respect to driving the expression of neuropeptide Y (NPY). This delivery system consists of an adeno-associated virus-derived (AAV) plasmid, pJDT95npy, encapsulated in reconstituted Sendai virosomes. pJDT95npy contains full length rat NPY cDNA inserted downstream from the P40 promoter in a cap-gene deleted AAV derived construct. The rep-sequences under control of the P5 and P19 promoters are intact. Virosomally encapsulated pJDT95npy drove the expression of NPY mRNAs, predominantly by P40. Total cellular NPY immunoreactivity and release in the presence of depolarization increased following pJDT95npy-transfection. Neither empty virosomes nor virosomes containing pJDT95 affected NPY mRNA expression or immunoreactivity. This study demonstrates that an AAVderived plasmid can drive exogenous gene expression in intact neurons after infusion by Sendai virosomes.

AB - A novel neuronal gene-delivery system was investigated in primary neuron-enriched cultures with respect to driving the expression of neuropeptide Y (NPY). This delivery system consists of an adeno-associated virus-derived (AAV) plasmid, pJDT95npy, encapsulated in reconstituted Sendai virosomes. pJDT95npy contains full length rat NPY cDNA inserted downstream from the P40 promoter in a cap-gene deleted AAV derived construct. The rep-sequences under control of the P5 and P19 promoters are intact. Virosomally encapsulated pJDT95npy drove the expression of NPY mRNAs, predominantly by P40. Total cellular NPY immunoreactivity and release in the presence of depolarization increased following pJDT95npy-transfection. Neither empty virosomes nor virosomes containing pJDT95 affected NPY mRNA expression or immunoreactivity. This study demonstrates that an AAVderived plasmid can drive exogenous gene expression in intact neurons after infusion by Sendai virosomes.

KW - Adeno-associated virus construct

KW - Neuropeptide Y

KW - Sendai virus

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Sendai virosomal infusion of an adeno-associated virus-derived construct containing neuropeptide Y into primary rat brain cultures

Abstract

Keywords

ASJC Scopus subject areas

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