Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles

Yan Li; Ying Lei Wong; Michelle Yueqi Lee; Qingxin Li; Qing Yin Wang; Julien Lescar; Pei Yong Shi; Cong Bao Kang

doi:10.1002/anie.201606609

Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles

Yan Li, Ying Lei Wong, Michelle Yueqi Lee, Qingxin Li, Qing Yin Wang, Julien Lescar, Pei Yong Shi, Cong Bao Kang

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Dengue virus nonstructural protein 4B (NS4B) is a membrane protein consisting of 248 residues with a crucial role in virus replication and interference with the host innate immunity. The dengue virus serotype 3 NS4B was reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. Backbone resonance assignment of NS4B was obtained using conventional solution NMR experiments. Further studies suggested that NS4B contained eleven helices and six of them form five potential transmembrane regions. This study provides atomic level information for an important drug target to control flavivirus infections.

Original language	English (US)
Pages (from-to)	12068-12072
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	55
Issue number	39
DOIs	https://doi.org/10.1002/anie.201606609
State	Published - Sep 19 2016

Keywords

NMR spectroscopy
NS4B
dengue virus
membrane protein
membrane topology

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.201606609

Cite this

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abstract = "Dengue virus nonstructural protein 4B (NS4B) is a membrane protein consisting of 248 residues with a crucial role in virus replication and interference with the host innate immunity. The dengue virus serotype 3 NS4B was reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. Backbone resonance assignment of NS4B was obtained using conventional solution NMR experiments. Further studies suggested that NS4B contained eleven helices and six of them form five potential transmembrane regions. This study provides atomic level information for an important drug target to control flavivirus infections.",

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AU - Li, Yan

AU - Wong, Ying Lei

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AU - Wang, Qing Yin

AU - Lescar, Julien

AU - Shi, Pei Yong

AU - Kang, Cong Bao

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Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles

Abstract

Keywords

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