Safety assessment of leaf curl virus resistant tomato developed using viral derived sequences

Abinav K. Singh, Shelly Praveen, Bhanu P. Singh, Anupam Varma, Naveen Arora

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Genetic engineering of food crops has significantly influenced the agricultural productivity over the past two decades. It has proved a valuable tool, offering crops with higher yields, improved nutritional quality, resistance against pesticides, herbicides and tolerance against abiotic stresses. However, the safety assessment of genetically engineered (GE) crops is prerequisite before introduction into human food chain. The present study was aimed to assess the toxicity and allergenicity of leaf curl virus resistant GE tomato compared to its wild-type species. Balb/c mice fed with genetically engineered or wild-type tomato did not show significant differences in growth, body weight (P > 0.05) and food consumption when compared with control mice. Values for serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase, urea and cholesterol were comparable in GE and wild-type tomato fed mice. Mice immunized with GE or wild-type tomato extract showed low IgE response. Lung histology of ovalbumin fed mice showed bronchoconstriction with eosinophilic infiltration whereas GE or wild-type tomato showed no cellular infiltration with normal airways. Genetically engineered and wild-type tomato sensitized mice demonstrated similar IL-4 release in splenic cell culture supernatant. GE and wild tomato extract on ELISA showed comparable IgE binding (P > 0.05) with food allergic patients' sera. In conclusion, genetically engineered tomato showed no toxicity in mice and allergenicity is similar to the wild-type tomato.

Original languageEnglish (US)
Pages (from-to)877-887
Number of pages11
JournalTransgenic Research
Issue number6
StatePublished - Nov 2009
Externally publishedYes


  • Allergenicity
  • Animal feeding studies
  • GE food safety
  • Toxicity
  • siRNA tomato

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Genetics
  • Agronomy and Crop Science


Dive into the research topics of 'Safety assessment of leaf curl virus resistant tomato developed using viral derived sequences'. Together they form a unique fingerprint.

Cite this