Ryanodine Receptor 1 Polymorphism Is Not Associated with Aneurysmal Subarachnoid Hemorrhage or its Clinical Sequelae

Philipp Hendrix; Paul M. Foreman; Mark R. Harrigan; Winfield S. Fisher; Nilesh A. Vyas; Robert H. Lipsky; Minkuan Lin; Beverly C. Walters; R. Shane Tubbs; Mohammadali M. Shoja; Jean Francois Pittet; Mali Mathru; Christoph J. Griessenauer

doi:10.1016/j.wneu.2016.12.132

Ryanodine Receptor 1 Polymorphism Is Not Associated with Aneurysmal Subarachnoid Hemorrhage or its Clinical Sequelae

Philipp Hendrix, Paul M. Foreman, Mark R. Harrigan, Winfield S. Fisher, Nilesh A. Vyas, Robert H. Lipsky, Minkuan Lin, Beverly C. Walters, R. Shane Tubbs, Mohammadali M. Shoja, Jean Francois Pittet, Mali Mathru, Christoph J. Griessenauer

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Objective The pathophysiologic mechanisms underlying cerebral vasospasm after aneurysmal subarachnoid hemorrhage (aSAH) remain poorly understand. Ryanodine receptors (RYR) are intracellular calcium channels involved in the regulation of vascular smooth muscle cells and cerebrovascular tone and diameter. Previous work reported an association between an RYR polymorphism and cerebral vasospasm. Here, we sought to assess the impact of that RYR polymorphism on aSAH and its clinical sequelae. Methods Blood samples from all patients enrolled in the CARAS (Cerebral Aneurysm Renin Angiotensin System) study were used for genetic evaluation. The RYR1 single nucleotide polymorphism (SNP) rs35364374 was detected using 5′exonuclease (Taqman) genotyping assays. Associations between the RYR1 polymorphism and aSAH and its clinical sequelae were analyzed. Results Samples from 149 patients with aSAH and 50 controls were available for analysis. Multivariable regression analysis did not show an association of RYR1 SNP rs35364374 with aSAH. Moreover, there was no association of RYR1 SNP rs35364374 with clinical vasospasm, delayed cerebral ischemia, functional outcome at discharge, or functional outcome at last follow-up. Conclusions Contrary to a previous report, the RYR1 SNP rs35364374 was not associated with aSAH or its clinical sequelae.

Original language	English (US)
Pages (from-to)	190-194
Number of pages	5
Journal	World Neurosurgery
Volume	100
DOIs	https://doi.org/10.1016/j.wneu.2016.12.132
State	Published - Apr 1 2017
Externally published	Yes

Keywords

Aneurysm
Clinical vasospasm
Delayed cerebral ischemia
Functional outcome
Polymorphism
Ryanodine receptor
Subarachnoid hemorrhage

ASJC Scopus subject areas

Surgery
Clinical Neurology

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10.1016/j.wneu.2016.12.132

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Hendrix, P., Foreman, P. M., Harrigan, M. R., Fisher, W. S., Vyas, N. A., Lipsky, R. H., Lin, M., Walters, B. C., Tubbs, R. S., Shoja, M. M., Pittet, J. F., Mathru, M., & Griessenauer, C. J. (2017). Ryanodine Receptor 1 Polymorphism Is Not Associated with Aneurysmal Subarachnoid Hemorrhage or its Clinical Sequelae. World Neurosurgery, 100, 190-194. https://doi.org/10.1016/j.wneu.2016.12.132

@article{9de0b92288c448a2b60873880b570cc9,

title = "Ryanodine Receptor 1 Polymorphism Is Not Associated with Aneurysmal Subarachnoid Hemorrhage or its Clinical Sequelae",

abstract = "Objective The pathophysiologic mechanisms underlying cerebral vasospasm after aneurysmal subarachnoid hemorrhage (aSAH) remain poorly understand. Ryanodine receptors (RYR) are intracellular calcium channels involved in the regulation of vascular smooth muscle cells and cerebrovascular tone and diameter. Previous work reported an association between an RYR polymorphism and cerebral vasospasm. Here, we sought to assess the impact of that RYR polymorphism on aSAH and its clinical sequelae. Methods Blood samples from all patients enrolled in the CARAS (Cerebral Aneurysm Renin Angiotensin System) study were used for genetic evaluation. The RYR1 single nucleotide polymorphism (SNP) rs35364374 was detected using 5′exonuclease (Taqman) genotyping assays. Associations between the RYR1 polymorphism and aSAH and its clinical sequelae were analyzed. Results Samples from 149 patients with aSAH and 50 controls were available for analysis. Multivariable regression analysis did not show an association of RYR1 SNP rs35364374 with aSAH. Moreover, there was no association of RYR1 SNP rs35364374 with clinical vasospasm, delayed cerebral ischemia, functional outcome at discharge, or functional outcome at last follow-up. Conclusions Contrary to a previous report, the RYR1 SNP rs35364374 was not associated with aSAH or its clinical sequelae.",

keywords = "Aneurysm, Clinical vasospasm, Delayed cerebral ischemia, Functional outcome, Polymorphism, Ryanodine receptor, Subarachnoid hemorrhage",

author = "Philipp Hendrix and Foreman, {Paul M.} and Harrigan, {Mark R.} and Fisher, {Winfield S.} and Vyas, {Nilesh A.} and Lipsky, {Robert H.} and Minkuan Lin and Walters, {Beverly C.} and Tubbs, {R. Shane} and Shoja, {Mohammadali M.} and Pittet, {Jean Francois} and Mali Mathru and Griessenauer, {Christoph J.}",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",

year = "2017",

month = apr,

day = "1",

doi = "10.1016/j.wneu.2016.12.132",

language = "English (US)",

volume = "100",

pages = "190--194",

journal = "World Neurosurgery",

issn = "1878-8750",

publisher = "Elsevier Inc.",

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TY - JOUR

T1 - Ryanodine Receptor 1 Polymorphism Is Not Associated with Aneurysmal Subarachnoid Hemorrhage or its Clinical Sequelae

AU - Hendrix, Philipp

AU - Foreman, Paul M.

AU - Harrigan, Mark R.

AU - Fisher, Winfield S.

AU - Vyas, Nilesh A.

AU - Lipsky, Robert H.

AU - Lin, Minkuan

AU - Walters, Beverly C.

AU - Tubbs, R. Shane

AU - Shoja, Mohammadali M.

AU - Pittet, Jean Francois

AU - Mathru, Mali

AU - Griessenauer, Christoph J.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Objective The pathophysiologic mechanisms underlying cerebral vasospasm after aneurysmal subarachnoid hemorrhage (aSAH) remain poorly understand. Ryanodine receptors (RYR) are intracellular calcium channels involved in the regulation of vascular smooth muscle cells and cerebrovascular tone and diameter. Previous work reported an association between an RYR polymorphism and cerebral vasospasm. Here, we sought to assess the impact of that RYR polymorphism on aSAH and its clinical sequelae. Methods Blood samples from all patients enrolled in the CARAS (Cerebral Aneurysm Renin Angiotensin System) study were used for genetic evaluation. The RYR1 single nucleotide polymorphism (SNP) rs35364374 was detected using 5′exonuclease (Taqman) genotyping assays. Associations between the RYR1 polymorphism and aSAH and its clinical sequelae were analyzed. Results Samples from 149 patients with aSAH and 50 controls were available for analysis. Multivariable regression analysis did not show an association of RYR1 SNP rs35364374 with aSAH. Moreover, there was no association of RYR1 SNP rs35364374 with clinical vasospasm, delayed cerebral ischemia, functional outcome at discharge, or functional outcome at last follow-up. Conclusions Contrary to a previous report, the RYR1 SNP rs35364374 was not associated with aSAH or its clinical sequelae.

AB - Objective The pathophysiologic mechanisms underlying cerebral vasospasm after aneurysmal subarachnoid hemorrhage (aSAH) remain poorly understand. Ryanodine receptors (RYR) are intracellular calcium channels involved in the regulation of vascular smooth muscle cells and cerebrovascular tone and diameter. Previous work reported an association between an RYR polymorphism and cerebral vasospasm. Here, we sought to assess the impact of that RYR polymorphism on aSAH and its clinical sequelae. Methods Blood samples from all patients enrolled in the CARAS (Cerebral Aneurysm Renin Angiotensin System) study were used for genetic evaluation. The RYR1 single nucleotide polymorphism (SNP) rs35364374 was detected using 5′exonuclease (Taqman) genotyping assays. Associations between the RYR1 polymorphism and aSAH and its clinical sequelae were analyzed. Results Samples from 149 patients with aSAH and 50 controls were available for analysis. Multivariable regression analysis did not show an association of RYR1 SNP rs35364374 with aSAH. Moreover, there was no association of RYR1 SNP rs35364374 with clinical vasospasm, delayed cerebral ischemia, functional outcome at discharge, or functional outcome at last follow-up. Conclusions Contrary to a previous report, the RYR1 SNP rs35364374 was not associated with aSAH or its clinical sequelae.

KW - Aneurysm

KW - Clinical vasospasm

KW - Delayed cerebral ischemia

KW - Functional outcome

KW - Polymorphism

KW - Ryanodine receptor

KW - Subarachnoid hemorrhage

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U2 - 10.1016/j.wneu.2016.12.132

DO - 10.1016/j.wneu.2016.12.132

M3 - Article

C2 - 28087430

AN - SCOPUS:85011388674

SN - 1878-8750

VL - 100

SP - 190

EP - 194

JO - World Neurosurgery

JF - World Neurosurgery

ER -

Ryanodine Receptor 1 Polymorphism Is Not Associated with Aneurysmal Subarachnoid Hemorrhage or its Clinical Sequelae

Abstract

Keywords

ASJC Scopus subject areas

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