Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication

Junhong Han; Hui Zhou; Bruce Horazdovsky; Kangling Zhang; Rui Ming Xu; Zhiguo Zhang

doi:10.1126/science.1133234

Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication

Junhong Han, Hui Zhou, Bruce Horazdovsky, Kangling Zhang, Rui Ming Xu, Zhiguo Zhang

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303 Scopus citations

Abstract

Acetylation of histone H3 lysine 56 (H3-K56) occurs in S phase, and cells lacking H3-K56 acetylation are sensitive to DNA-damaging agents. However, the histone acetyltransferase (HAT) that catalyzes global H3-K56 acetylation has not been found. Here we show that regulation of Ty1 transposition gene product 109 (Rtt109) is an H3-K56 HAT. Cells lacking Rtt109 or expressing rtt109 mutants with alterations at a conserved aspartate residue lose H3-K56 acetylation and exhibit increased sensitivity toward genotoxic agents, as well as elevated levels of spontaneous chromosome breaks. Thus, Rtt109, which shares no sequence homology with any other known HATs, is a unique HAT that acetylates H3-K56.

Original language	English (US)
Pages (from-to)	653-655
Number of pages	3
Journal	Science
Volume	315
Issue number	5812
DOIs	https://doi.org/10.1126/science.1133234
State	Published - Feb 2 2007
Externally published	Yes

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title = "Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication",

abstract = "Acetylation of histone H3 lysine 56 (H3-K56) occurs in S phase, and cells lacking H3-K56 acetylation are sensitive to DNA-damaging agents. However, the histone acetyltransferase (HAT) that catalyzes global H3-K56 acetylation has not been found. Here we show that regulation of Ty1 transposition gene product 109 (Rtt109) is an H3-K56 HAT. Cells lacking Rtt109 or expressing rtt109 mutants with alterations at a conserved aspartate residue lose H3-K56 acetylation and exhibit increased sensitivity toward genotoxic agents, as well as elevated levels of spontaneous chromosome breaks. Thus, Rtt109, which shares no sequence homology with any other known HATs, is a unique HAT that acetylates H3-K56.",

author = "Junhong Han and Hui Zhou and Bruce Horazdovsky and Kangling Zhang and Xu, {Rui Ming} and Zhiguo Zhang",

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T1 - Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication

AU - Han, Junhong

AU - Zhou, Hui

AU - Horazdovsky, Bruce

AU - Zhang, Kangling

AU - Xu, Rui Ming

AU - Zhang, Zhiguo

PY - 2007/2/2

Y1 - 2007/2/2

N2 - Acetylation of histone H3 lysine 56 (H3-K56) occurs in S phase, and cells lacking H3-K56 acetylation are sensitive to DNA-damaging agents. However, the histone acetyltransferase (HAT) that catalyzes global H3-K56 acetylation has not been found. Here we show that regulation of Ty1 transposition gene product 109 (Rtt109) is an H3-K56 HAT. Cells lacking Rtt109 or expressing rtt109 mutants with alterations at a conserved aspartate residue lose H3-K56 acetylation and exhibit increased sensitivity toward genotoxic agents, as well as elevated levels of spontaneous chromosome breaks. Thus, Rtt109, which shares no sequence homology with any other known HATs, is a unique HAT that acetylates H3-K56.

AB - Acetylation of histone H3 lysine 56 (H3-K56) occurs in S phase, and cells lacking H3-K56 acetylation are sensitive to DNA-damaging agents. However, the histone acetyltransferase (HAT) that catalyzes global H3-K56 acetylation has not been found. Here we show that regulation of Ty1 transposition gene product 109 (Rtt109) is an H3-K56 HAT. Cells lacking Rtt109 or expressing rtt109 mutants with alterations at a conserved aspartate residue lose H3-K56 acetylation and exhibit increased sensitivity toward genotoxic agents, as well as elevated levels of spontaneous chromosome breaks. Thus, Rtt109, which shares no sequence homology with any other known HATs, is a unique HAT that acetylates H3-K56.

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Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication

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