Role of Litopenaeus vannamei Yin Yang 1 in the regulation of the white spot syndrome virus immediate early gene ie1

Ping Han Huang, Ting Yi Huang, Pei Si Cai, Li Kwan Chang

Research output: Contribution to journalArticlepeer-review

Abstract

Yin Yang 1 (YY1) is a multifunctional zinc finger transcription factor that regulates many key cellular processes. In this study, we report the cloning of YY1 from Litopenaeus vannamei shrimp (LvYY1). This study shows that LvYY1 is ubiquitously expressed in shrimp tissues, and knockdown of LvYY1 expression by doublestranded RNA (dsRNA) injection in white spot syndrome virus (WSSV)-infected shrimp reduced both mRNA levels of the WSSV immediate early gene ie1 as well as overall copy numbers of the WSSV genome. The cumulative mortality rate of infected shrimp also declined with LvYY1 dsRNA injection. Using an insect cell model, we observed that LvYY1 activates ie1 expression, and a mutation introduced into the ie1 promoter subsequently repressed this capability. Moreover, reporter assay results suggested that LvYY1 is involved in basal transcriptional regulation via an interaction with L. vannamei TATA-binding protein (LvTBP). Electrophoretic mobility shift assay (EMSA) results further indicated that LvYY1 binds to a YY1-binding site in the region between positions -119 and -126 in the ie1 promoter. Chromatin immunoprecipitation analysis also confirmed that LvYY1 binds to the ie1 promoter in WSSVinfected shrimp. Taken together, these results indicate that WSSV uses host LvYY1 to enhance ie1 expression via a YY1-binding site and the TATA box in the ie1 promoter, thereby facilitating lytic activation and viral replication.

Original languageEnglish (US)
Article numbere02314-16
JournalJournal of virology
Volume91
Issue number6
DOIs
StatePublished - 2017
Externally publishedYes

Keywords

  • Ie1
  • Litopenaeus vannamei
  • TATA-binding protein
  • Transcriptional regulation
  • WSSV
  • Yin Yang 1

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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