Role of ENPP1 on adipocyte maturation

Jian Liang, Mingui Fu, Ester Ciociola, Manisha Chandalia, Nicola Abate

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


Background. It is recognized that the ability of adipose tissue to expand in response to energy excess, i.e. adipocyte maturation, is important in determining systemic abnormalities in glucose and lipid metabolism. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1, also known as PC-1) has been recently reported to be involved in the pathogenesis of insulin resistance and related.However, its role on adipose tissue physiology as a mechanism of systemic insulin resistance is not understood. This study was performed to evaluate whether ENPP1 is regulated during adipogenesis and whether over-expression in adipocytes can affect adipocyte maturation, a potential novel mechanism of ENPP1-related insulin resistance. Methodology/Principal Findings. ENPP1 expression was found down-requlated during 3T3-L1 maturation, and over-expression of human ENPP1 in 3T3-L1 (pQCXIP-ENPP1 vector) resulted in adipocyte insulin resistance and in defective adipocyte maturation. Adipocyte maturation was more efficient in mesenchymal embryonal cells from ENPP1 knockout mice than from wild-type. Conclusions. We identity ENPP1 as a novel mechanism of defective adipocyte maturation. This mechanism could contribute to the pathogenesis of insulin resistance in absence of obesity.

Original languageEnglish (US)
Article numbere882
JournalPloS one
Issue number9
StatePublished - Sep 12 2007
Externally publishedYes

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Role of ENPP1 on adipocyte maturation'. Together they form a unique fingerprint.

Cite this