Role of DNA repair in ethyl methanesulfonate-induced mutagenesis in saccharomyces cerevisiae

Louise Prakash; David Higgins

doi:10.1093/carcin/3.4.439

Role of DNA repair in ethyl methanesulfonate-induced mutagenesis in saccharomyces cerevisiae

Louise Prakash, David Higgins

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Ethyl methanesulfonate (EMS)-induced reversion of two sites which revert preferentially by GC to AT transitions, cycl-131 and cycl-115, has been examined in readiation sensitive, rad, mutants of yeast belonging to the rad52 epistasis group. The rad50, rad51, rad52, rad54 and rad56 mutants showed reduced reversion of both tester sites when stationary phase diploid cells were treated with EMS. No correlation was found between EMS-induced reversion and EMS-induced homologous mitotic intragenic recombination. Survival of rad6 rad52 double mutants following EMS treatment indicates that there is one epistasis group for the repair of EMS-induced lethal damage in yeast. A model involving misrepair mutagenesis of specific lesions is proposed to account for the experimental results.

Original language	English (US)
Pages (from-to)	439-444
Number of pages	6
Journal	Carcinogenesis
Volume	3
Issue number	4
DOIs	https://doi.org/10.1093/carcin/3.4.439
State	Published - 1982
Externally published	Yes

ASJC Scopus subject areas

Cancer Research

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title = "Role of DNA repair in ethyl methanesulfonate-induced mutagenesis in saccharomyces cerevisiae",

abstract = "Ethyl methanesulfonate (EMS)-induced reversion of two sites which revert preferentially by GC to AT transitions, cycl-131 and cycl-115, has been examined in readiation sensitive, rad, mutants of yeast belonging to the rad52 epistasis group. The rad50, rad51, rad52, rad54 and rad56 mutants showed reduced reversion of both tester sites when stationary phase diploid cells were treated with EMS. No correlation was found between EMS-induced reversion and EMS-induced homologous mitotic intragenic recombination. Survival of rad6 rad52 double mutants following EMS treatment indicates that there is one epistasis group for the repair of EMS-induced lethal damage in yeast. A model involving misrepair mutagenesis of specific lesions is proposed to account for the experimental results.",

author = "Louise Prakash and David Higgins",

note = "Funding Information: We thank Patricia Terho and Patricia Taillon-Miller for technical assistance. This paper is based on work performed partially under grant ES-02433 from NIEHS and Contract Number DE-AC02-76EV03490 with the U.S. Department of Energy at the University of Rochester Department of Radiation Biology and Biophysics and has been assigned report no. UR-3490-2106.",

year = "1982",

doi = "10.1093/carcin/3.4.439",

language = "English (US)",

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pages = "439--444",

journal = "Carcinogenesis",

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publisher = "Oxford University Press",

number = "4",

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T1 - Role of DNA repair in ethyl methanesulfonate-induced mutagenesis in saccharomyces cerevisiae

AU - Prakash, Louise

AU - Higgins, David

N1 - Funding Information: We thank Patricia Terho and Patricia Taillon-Miller for technical assistance. This paper is based on work performed partially under grant ES-02433 from NIEHS and Contract Number DE-AC02-76EV03490 with the U.S. Department of Energy at the University of Rochester Department of Radiation Biology and Biophysics and has been assigned report no. UR-3490-2106.

PY - 1982

Y1 - 1982

N2 - Ethyl methanesulfonate (EMS)-induced reversion of two sites which revert preferentially by GC to AT transitions, cycl-131 and cycl-115, has been examined in readiation sensitive, rad, mutants of yeast belonging to the rad52 epistasis group. The rad50, rad51, rad52, rad54 and rad56 mutants showed reduced reversion of both tester sites when stationary phase diploid cells were treated with EMS. No correlation was found between EMS-induced reversion and EMS-induced homologous mitotic intragenic recombination. Survival of rad6 rad52 double mutants following EMS treatment indicates that there is one epistasis group for the repair of EMS-induced lethal damage in yeast. A model involving misrepair mutagenesis of specific lesions is proposed to account for the experimental results.

AB - Ethyl methanesulfonate (EMS)-induced reversion of two sites which revert preferentially by GC to AT transitions, cycl-131 and cycl-115, has been examined in readiation sensitive, rad, mutants of yeast belonging to the rad52 epistasis group. The rad50, rad51, rad52, rad54 and rad56 mutants showed reduced reversion of both tester sites when stationary phase diploid cells were treated with EMS. No correlation was found between EMS-induced reversion and EMS-induced homologous mitotic intragenic recombination. Survival of rad6 rad52 double mutants following EMS treatment indicates that there is one epistasis group for the repair of EMS-induced lethal damage in yeast. A model involving misrepair mutagenesis of specific lesions is proposed to account for the experimental results.

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JO - Carcinogenesis

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Role of DNA repair in ethyl methanesulfonate-induced mutagenesis in saccharomyces cerevisiae

Abstract

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